自噬相关蛋白Atg5在BPA所致肝脏脂质沉积中的作用研究

发布时间：2018-06-14 14:51

本文选题：双酚A + 脂质沉积　；参考：《重庆医科大学学报》2015年03期

【摘要】：目的:观察自噬相关蛋白Atg5是否介导双酚A(bisphenol A,BPA)所致的肝脏脂质沉积。方法:将Hep G2肝细胞分为对照组、BPA不同浓度(1、10μmol/L)干预组、阳性对照组即200μmol/L油酸(oleic acid,OA)联合400μmol/L软脂酸(Palmitic acid,PA),分别给予上述不同干预24 h。采用化学酶促-比色法定量检测细胞内甘油三酯(triglyceride,TG)含量,油红O染色观察细胞内脂滴沉积情况。Real-time PCR及Western blot、免疫荧光方法检测在不同干预情况下Hep G2细胞内Atg5的表达水平。结果:1不同浓度BPA干预后,Hep G2细胞内TG含量均有不同程度的升高,其中10μmol/L BPA组升高具有统计学意义(0.197±0.033;0.094±0.014,P=0.031);2油红O染色结果与TG定量一致。3Real-time PCR结果表明,与对照组相比10μmol/L BPA组Atg5 m RNA表达并无明显变化(0.93±0.088;1.017 5±0.062 4,t=0.811,P=0.428);Western blot结果显示与对照组相比10μmol/L BPA组其蛋白表达明显减少(0.519±0.059;1,t=0.8101,P=0.000);免疫荧光进一步检测发现,与对照组相比10μmol/L BPA组Atg5荧光小点数量明显减少。结论:自噬相关蛋白Atg5可能介导BPA所致的肝脏脂质沉积的发生。
[Abstract]:Aim: to investigate whether autophagy associated protein (Atg5) mediates hepatic lipid deposition induced by bisphenol A (BPA). Methods: HepG2 hepatocytes were divided into two groups: the control group treated with 10 渭 mol / L BPA and the positive control group (200 渭 mol / L oleic acididine) combined with 400 渭 mol / L palmitic acididine for 24 h. The content of triglyceride-triglyceride@@ Results the content of TG in Hep G2 cells increased in different degree after the intervention of BPA at different concentration of BPA. The increase of TG in 10 渭 mol / L BPA group was statistically significant (0.197 卤0.033 卤0.094 卤0.014). The results of oil red O staining were in agreement with the quantitative analysis of TG. 3 Real-time PCR results showed that the content of TG in Hep G2 cells was significantly higher than that in 10 渭 mol / L BPA group. There was no significant change in Atg5 mRNA expression in 10 渭 mol / L BPA group compared with the control group. The expression of Atg5 mRNA in 10 渭 mol / L BPA group was significantly lower than that in the control group (0. 519 卤0. 0591 t 0. 8101 P0. 000), and the results of immunofluorescence further detection showed that the protein expression of 10 渭 mol / L BPA group was significantly lower than that of the control group (0. 519 卤0. 0591 t 0. 8101 P0. 000). Compared with the control group, the number of Atg5 fluorescent spots in 10 渭 mol / L BPA group was significantly reduced. Conclusion: autophagy associated protein Atg5 may mediate lipids deposition in liver.
【作者单位】：重庆医科大学附属第一医院内分泌科;
【基金】：国家自然科学基金资助项目(编号:81370954、81170751、81300486) 国家临床重点专科建设项目
【分类号】：R575.5

【共引文献】