乐复能对实验性结肠炎小鼠模型肠粘膜内IL-8、MCP-1表达的影响

发布时间：2018-06-14 22:25

本文选题：乐复能 + 美沙拉嗪　；参考：《中南大学》2014年硕士论文

【摘要】：目的研究观察乐复能(novaferon)对葡聚糖硫酸钠(DSS)诱导的实验性结肠炎小鼠模型肠粘膜内趋化因子IL-8和MCP-1表达的影响。方法将70只Balb/C雌性小鼠,SPF级,6-8周龄,18-22g,分成7组,每组10只。分别为正常组,模型组,乐复能高、中、低剂量组、美沙拉嗪组及泼尼松组。予以4%DSS液自由饮用7天造实验性结肠炎小鼠模型。造模第二天开始分别给予正常组和模型组无菌生理盐水灌胃,美沙拉嗪组、泼尼松组分别给予美沙拉嗪和泼尼松灌胃,乐复能各剂量组分别给予0.1ug/ml、0.2ug/ml、0.3ug/ml乐复能腹腔注射,0.2m1/次,以上处理均为每日一次。造模第八天处死所有小鼠。实验过程中观察小鼠的体重、活动情况、大便隐血情况,对小鼠进行DAI评分,处死小鼠取得标本后观察组织学损伤评分,免疫组化检测小鼠肠粘膜内IL-8、MCP-1的表达。结果 1、除正常组小鼠外,其他用DSS干预的小鼠均不同程度的出现便血、体重下降、活动度减退的情况,小鼠的DAI积分逐渐增加,经乐复能、美沙拉嗪、泼尼松处理后的小鼠的一般情况、DAI积分、组织学损伤评分有所下降。 2、和模型组相比较,乐复能高剂量组、美沙拉嗪组、泼尼松组实验性结肠炎小鼠模型肠粘膜内IL-8、MCP-1的表达下降(P0.05),有统计学意义。结论葡聚糖硫酸钠(DSS)诱导的实验性结肠炎小鼠模型和溃疡性结肠炎(UC)的临床症状及组织学特点类似；乐复能、美沙拉嗪、泼尼松可以下调实验性结肠炎小鼠模型肠粘膜内IL-8、MCP-1的表达。
[Abstract]:Objective to investigate the effect of Lefongneng Novaferon on the expression of IL-8 and MCP-1 in intestinal mucosa of mice with experimental colitis induced by dextran sodium sulfate (DSS). Methods 70 Balb / C female mice were divided into 7 groups with 10 rats in each group. They were normal group, model group, high, middle and low dose group, mesalazine group and prednisone group. The mice model of experimental colitis was established with 4 DSS solution for 7 days. On the second day of model making, the normal group and the model group were given aseptic saline, the mesalazine group and prednisone group were given intragastric administration of mesalazine and prednisone respectively, and each dose group was given 0.1ugml / ml 0.2ugP / ml Loflon intraperitoneal injection of 0.2m1g / ml, respectively. The above treatment is once a day. On the eighth day, all the mice were killed. The weight, activity and fecal occult blood of the mice were observed during the experiment. Dai score was used to evaluate the mice. The histological injury score was observed after the mice were killed. The expression of IL-8 and MCP-1 in the intestinal mucosa of the mice was detected by immunohistochemistry. Results 1 except in the normal group, the other mice treated with DSS had hematochezia, decreased body weight and decreased activity, the Dai score of the mice increased gradually, and the rats were treated with Leferin and mesalazine. After prednisone treatment, Dai score and histological injury score were decreased in mice treated with prednisone. 2Compared with the model group, the high dose group of loflon, the group of mesalazine, In prednisone group, the expression of IL-8 and MCP-1 in intestinal mucosa of experimental colitis mice was decreased (P 0.05). Conclusion the clinical symptoms and histological characteristics of experimental colitis mice induced by dextran sodium sulfate (DSS) and ulcerative colitis were similar. Prednisone could down-regulate the expression of IL-8 and MCP-1 in intestinal mucosa of experimental colitis mice.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R574.62

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