GYY4137对小鼠原代肝细胞胞质内脂质分解的影响

发布时间：2018-06-17 00:41

本文选题：硫化氢 + 胞质内脂质分解　；参考：《中国病理生理杂志》2017年08期

【摘要】：目的:探讨新合成的硫化氢(H2S)供体GYY4137对小鼠原代肝脂肪变性细胞胞质内脂质分解的影响。方法:体外用油酸诱导肝细胞脂肪变性模型。采用两步原位灌流法分离C57BL/6小鼠原代肝细胞并分为4组:对照组用正常培养液培养54 h;模型组用含1.2 mmol/L油酸(溶于10%BSA)的培养液培养48 h,再用RPMI-1640培养液培养6 h;H2S组和DL-炔丙基甘氨酸(PAG;胱硫醚γ-裂解酶抑制剂,抑制H2S合成)组则用含有1.2mmol/L油酸的培养液培养48 h,再用无血清无酚红的RPMI-1640培养液(分别给予含有1 mmol/L GYY4137和200μmol/L PAG)培养6 h。测量细胞甘油释放量及胞内激素敏感性脂肪酶(HSL)的蛋白表达量。结果:和模型组相比,H_2S组培养液中甘油释放量和磷酸化HSL(p-HSL)蛋白表达水平明显降低,而PAG组又明显升高。结论:在小鼠原代脂肪变性肝细胞中,GYY4137可能通过抑制HSL的磷酸化水平而减少胞质内脂质分解。
[Abstract]:Aim: to investigate the effect of GYY4137, a newly synthesized H _ 2S donor, on lipid decomposition in primary hepatic steatosis cells of mice. Methods: fatty degeneration of hepatocytes was induced by oleic acid in vitro. C57BL / 6 mouse primary hepatocytes were isolated by two-step in situ perfusion and divided into four groups: the control group was cultured in normal medium for 54 h, the model group was cultured in a medium containing 1.2 mmol / L oleic acid (dissolved in 10 BSAs) for 48 h, and then cultured in RPMI-1640 medium for 6 h in H2S group. And DL-propargyl glycine, PAG; cystathionine 纬 -lyase inhibitor, In the group of inhibiting H2S synthesis, 1.2mmol-L oleic acid was used for 48 h, and then RPMI-1640 (containing 1 mmol / L GYY4137 and 200 渭 mol / L pal) was used for 6 h. The amount of glycerol release and the protein expression of intracellular hormone sensitive lipase (HSL) were measured. Results: compared with the model group, the level of glycerol release and phosphorylated HSL-p-HSL-HSL-HSL-HSL-HSL-HSL-HSL-P protein was significantly decreased in HS-treated group, while that in PAG group was significantly higher than that in the model group. Conclusion: GYY4137 may reduce lipid decomposition by inhibiting the phosphorylation level of HSL in primary adipose degeneration hepatocytes of mice.
【作者单位】：河南大学基础医学院;
【基金】：国家自然科学基金资助项目(No.3130084) 河南省高校重点科研项目(No.16A310001)
【分类号】：R575.5

【参考文献】