JNK信号通路在脂多糖诱导的小鼠急性肝损伤中的表达研究

发布时间：2018-06-17 21:51

本文选题：脂多糖 + 急性肝损伤　；参考：《中国现代医学杂志》2015年20期

【摘要】：目的观察脂多糖(LPS)诱导的小鼠急性肝损伤模型肝组织中JNK信号通路(p-JNK和p-c-Jun)的表达变化,以探讨JNK信号通路在急性肝损伤中的作用和意义。方法将小鼠随机分为对照组和LPS腹腔注射诱导的急性肝损伤模型组,分别于给药后1、2、4和30 h处死小鼠,检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平。HE染色观察肝脏组织的病理变化。免疫组织化学染色、Western blot检测肝脏组织p-JNK和p-c-Jun蛋白的表达部位及强度。酶联免疫吸附(ELISA)法检测血清肿瘤坏死因子-α(TNF-α)及白介素1β(IL-1β)水平。结果 LPS腹腔注射后可导致小鼠血清ALT及AST水平升高,肝组织病理损伤进行性加重,p-JNK和p-c-Jun在肝细胞核大量表达。与对照组比较,模型组小鼠p-JNK和p-c-Jun蛋白水平于1 h时显著升高并达峰值,此后逐渐下降,血清TNF-α及IL-1β水平在注射LPS 1 h后明显升高,4 h达峰值后逐渐下降。结论 JNK信号通路的活化在LPS诱导的小鼠急性肝损伤的发病过程中起重要作用,JNK信号通路可通过诱导TNF-α和IL-1β的表达参与急性肝损伤的发生与发展。
[Abstract]:Objective to investigate the expression of JNK signaling pathway (p-JNK and p-c-Jun) in liver tissue of lipopolysaccharide (LPS) -induced acute liver injury in mice, and to explore the role and significance of JNK signal pathway in acute liver injury. Methods mice were randomly divided into control group and acute hepatic injury model group induced by intraperitoneal injection of LPS. The mice were killed at 1h and 30h after administration of lipopolysaccharide (LPS). The levels of serum alanine aminotransferase (alt) and aspartate transaminase (AST) were detected. The pathological changes of liver tissue were observed by HE staining. The expression site and intensity of p-JNK and p-c-Jun protein in liver tissue were detected by immunohistochemical staining and Western blot. Serum tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 尾 (IL-1 尾) were detected by Elisa. Results the levels of alt and AST in serum of mice were increased after intraperitoneal injection of LPS, and the expression of p-JNK and p-c-Jun in liver nuclei was increased gradually. Compared with the control group, the levels of p-JNK and p-c-Jun protein in the model group increased significantly and reached the peak at 1 h, then decreased gradually, and the levels of serum TNF- 伪 and IL-1 尾 increased significantly at 1 h after LPS injection and gradually decreased at 4 h after LPS injection. Conclusion the activation of JNK signaling pathway plays an important role in the pathogenesis of LPS-induced acute liver injury in mice. JNK signaling pathway may participate in the occurrence and development of acute liver injury by inducing the expression of TNF- 伪 and IL-1 尾.
【作者单位】：泸州医学院附属医院肾内科;泸州医学院附属医院肝胆外科;
【基金】：四川省科技厅资助课题(No:2014JY0068)
【分类号】：R575

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