幽门螺杆菌感染对LAIR-1影响的实验研究

发布时间：2018-06-17 20:37

本文选题：幽门螺杆菌 + CBL/小鼠　；参考：《中国病原生物学杂志》2015年08期

【摘要】：目的通过构建小鼠感染模型,研究幽门螺杆菌(Hp)感染对白细胞相关免疫球蛋白样受体-1(LAIR-1)表达的影响。方法将32只C57BL/6小鼠随机分为对照组和感染组。感染组通过Hp菌液灌胃建立小鼠感染模型,对照组以PBS灌胃。6周后处死小鼠,采用胃黏膜分离培养、革兰染色、PCR及生化反应检查Hp定植情况,采用流式细胞术检测小鼠外周血单个核细胞(PBMC)中单核细胞和淋巴细胞表面LAIR-1的表达。结果感染组小鼠Hp感染率为91.3%(21/23)。感染组和对照组小鼠PBMC中单核细胞比例分别为(22.85±3.56)%和(49.85±0.35)%,淋巴细胞比例分别为(40.93±10.05)%和(30.15±0.35)%,差异均有统计学意义(t=5.845和2.137,P0.05)。感染组和对照组小鼠PBMC表面LAIR-1表达率分别为(70.16±7.75)%和(83.4±0.1)%,淋巴细胞表面LAIR-1表达率分别为(56.81±11.37)%和(72.3±0.6)%,差异均有统计学意义(t=3.761和2.491,P0.05)。结论 Hp感染下调了小鼠PBMC中单核细胞及LAIR-1的表达,上调淋巴细胞比例但下调其LAIR-1的表达。
[Abstract]:Objective to study the effect of Helicobacter pylori (Hp) infection on the expression of leukocyte related immunoglobulin like receptor -1 (LAIR-1) by constructing a mouse infection model. Methods 32 C57BL/6 mice were randomly divided into the control group and the infection group. The infection group was injected with Hp bacteria to establish the mice infection model, and the control group died of the mice after.6 weeks after PBS gavage. The expression of LAIR-1 in mononuclear cells and lymphocytes in peripheral blood mononuclear cells (PBMC) of mice was detected by flow cytometry. The infection rate of Hp in infected mice was 91.3% (21/23). The proportion of mononuclear cells in PBMC of the infected group and the control group was respectively (PBMC), and the ratio of mononuclear cells in PBMC of the infected group and the control group was respectively (Hp). 22.85 + 3.56% and (49.85 + 0.35)%, and the proportion of lymphocytes were (40.93 + 10.05)% and (30.15 + 0.35)% respectively. The difference was statistically significant (t=5.845 and 2.137, P0.05). The LAIR-1 expression rate of PBMC on the surface of the infected and control mice was (70.16 + 7.75)% and (83.4 + 0.1)% respectively, and the LAIR-1 expression rate of the lymphocyte surface was (49.85)% and The difference was statistically significant (t=3.761 and 2.491, P0.05). Conclusion Hp infection downregulated the expression of mononuclear cells and LAIR-1 in mouse PBMC, up - regulation of lymphocyte proportion but down the expression of LAIR-1.
【作者单位】：滨州医学院病原生物学教研室;
【基金】：国家自然科学基金项目(No.81471561) 教育部"新世纪优秀人才支持计划"(No.NCET-11-1026)
【分类号】：R573

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