茶多酚在四氯化碳所致大鼠肝硬化中的作用

发布时间：2018-06-22 01:12

本文选题：肝硬化 + 门静脉高压　；参考：《蚌埠医学院》2017年硕士论文

【摘要】：目的:观察茶多酚(TP)对CC14所致大鼠肝硬化的保护作用,并探讨其可能的作用机制。方法:雄性SD大鼠40只,随机分为4组,正常对照组(N组)、正常加茶多酚组(N+TP组)、肝硬化组(PHT组)、茶多酚干预组(PHT+TP组)。PHT组及PHT+TP组分别按0.3ml/100g皮下注射CC14,每周2次,其余两组皮下注射等量生理盐水。在造模12周后,N+TP组和PHT+TP组均用茶多酚[150mg/(kg.d)]灌胃治疗。16周末检测各项指标(血清ALT、AST,氧化应激指标,平均动脉压,门静脉压力),处死各组大鼠,留取肝脏组织,观察各组大鼠肝组织病理改变(肝组织HE染色),测定α-SMA,并比较各组间的区别有无统计学意义。结果:茶多酚干预组与肝硬化组相比,茶多酚治疗组的肝纤维化大鼠肝脏中α-SMA水平及ALT和AST表达均降低,组织病理观察显示茶多酚干预组肝硬化程度比肝硬化组轻,门脉压力下降。结论:茶多酚能减轻CC14诱导大鼠发生的肝损伤与肝硬化,其机制可能为通过降低肝组织氧化应激水平而发挥护肝作用。
[Abstract]:Aim: to observe the protective effect of tea polyphenols (TP) on CC14 induced liver cirrhosis in rats and to explore its possible mechanism. Methods: forty male Sprague-Dawley rats were randomly divided into 4 groups: normal control group (N group), normal tea polyphenol group (N TP group), cirrhosis group (PHT group), tea polyphenol intervention group (PHT TP group). The other two groups were subcutaneously injected with the same amount of normal saline. After 12 weeks of model making, the rats in the N TP group and the 150mg/ TP group were treated with 150mg/ (kg.d). At the end of week 16, the serum alt AST, oxidative stress index, mean arterial pressure and portal vein pressure were measured. The rats in each group were killed and the liver tissue was taken. The pathological changes of liver tissue (HE staining) and 伪 -SMAwere observed in each group, and the difference between each group was statistically significant. Results: the level of 伪 -SMA and the expression of alt and AST in the liver of the tea polyphenol treated group were lower than that of the cirrhosis group. Histopathological observation showed that the liver cirrhosis degree in the tea polyphenol treated group was less than that in the cirrhosis group. Portal pressure decreased. Conclusion: tea polyphenols can attenuate the liver injury and cirrhosis induced by CC14 in rats. The mechanism of tea polyphenols may play a protective role by reducing the level of oxidative stress in liver tissue.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R575.2

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