肝损伤大鼠血清诱导培养脐带间充质干细胞向肝样细胞的分化

发布时间：2018-07-03 08:35

本文选题：脐带 + 间质干细胞　；参考：《中国组织工程研究》2015年23期

【摘要】：背景:脐带间充质干细胞来源于新生个体脐带组织,来源广泛,无社会伦理学因素制约,有望代替骨髓间充质干细胞成为细胞移植和再生医学的理想种子细胞。目的:探讨肝衰竭大鼠血清对人脐带间充质干细胞肝向诱导分化作用,为临床上利用脐带间充质干细胞治疗终末期肝病提供实验依据。方法:腹腔注射10%四氯化碳溶液建立急性肝损伤大鼠模型,对照组腹腔注射等剂量的大豆油,48 h后腹主动脉取血离心分离血清。取第3代人脐带间充质干细胞,分别用体积分数为20%肝损伤大鼠血清和体积分数为20%胎牛血清诱导培养,观察诱导前后人脐带间充质干细胞形态学改变,检测培养液上清甲胎蛋白、白蛋白水平。结果与结论:肝损伤大鼠血清培养第1天细胞形态变化不大,呈梭形,仍呈编织状或漩涡状生长,第2天细胞呈短梭形,第3天细胞呈类圆形,第4天呈圆形,并有极少量细胞漂浮。肝损伤大鼠血清培养人脐带间充质干细胞4 d,其上清液白蛋白水平较诱导前和对照组均有升高趋势(P0.001),甲胎蛋白水平无明显变化。结果表明肝损伤大鼠血清可使脐带间充质干细胞向肝样细胞分化。
[Abstract]:Background: umbilical cord mesenchymal stem cells (UMSCs) are derived from newborn human umbilical cord tissues and have no social ethical constraints. They are expected to replace bone marrow mesenchymal stem cells (BMSCs) as ideal seed cells for cell transplantation and regenerative medicine. Objective: to investigate the effect of serum from rats with liver failure on the differentiation of human umbilical cord mesenchymal stem cells into liver, and to provide experimental evidence for the treatment of end-stage liver disease with umbilical cord mesenchymal stem cells. Methods: the rat model of acute liver injury was established by intraperitoneal injection of 10% carbon tetrachloride solution. The third generation of human umbilical cord mesenchymal stem cells were cultured with 20% rat serum and 20% fetal bovine serum respectively. The morphological changes of human umbilical cord mesenchymal stem cells were observed before and after induction. The levels of Albumin and alpha-fetoprotein in supernatant were measured. Results and conclusion: on the first day after liver injury, the cell morphology of the rat serum was not changed much, but it was still spindle-shaped or whirlpool. On the second day, the cells were short fusiform, on the 3rd day the cells were round, and on the 4th day the cells were round. And there are very few cells floating. The level of albumin in the supernatant of cultured human umbilical cord mesenchymal stem cells (HMSCs) cultured in serum of rats with liver injury showed a trend of increase (P0.001) and no significant change in alpha-fetoprotein level as compared with that before induction and control group (P0.001). The results showed that rat serum could differentiate umbilical cord mesenchymal stem cells into hepatocyte-like cells.
【作者单位】：中南大学湘雅二医院肝病中心;
【分类号】：R575

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