基于分光光度法测定血液中非那西丁与扑热息痛含量的肝脏储备功能评估

发布时间：2018-07-04 07:51

本文选题：肝脏储备功能 + 分光光度技术　；参考：《山东医药》2015年04期

【摘要】：目的建立基于分光光度法测定非那西丁与扑热息痛含量的肝脏储备功能评估方法。方法筛选分光光度测定的显色体系,确定最大吸收波长,分析不同因素对显色体系的影响,优化非那西丁与扑热息痛水解的最佳条件,最后对技术体系进行应用性验证。结果建立了利用分光光度测定样品中非那西丁和扑热息痛含量的技术体系,即样品加入3 mol/L盐酸水解30 min,加入0.02%1,2-萘醌-4-磺酸钠、1%十六烷基三甲基溴化铵及2%Na OH(或3%Na2CO3)(比例为1∶6∶1∶2或3),分别于500 nm和570 nm下测定吸光值,计算各自浓度。该技术测定血浆样品中非那西丁与扑热息痛时的分辨率和重复性与高效液相色谱法相当。结论利用分光光度技术测定血液中非那西丁与扑热息痛含量,二者比值具有评估肝脏储备功能价值。
[Abstract]:Objective to establish a method for evaluating liver reserve function based on spectrophotometric determination of phenacetin and paracetamol. Methods the color reaction system for spectrophotometric determination was screened, the maximum absorption wavelength was determined, the influence of different factors on the color reaction system was analyzed, the optimum conditions for the hydrolysis of phenacetin and paracetamol were optimized, and the application of the technical system was verified. Results A technical system for the determination of phenacetin and paracetamol in samples by spectrophotometry was established. That is to say, the sample was hydrolyzed with 3 mol / L hydrochloric acid for 30 min, and the absorptivity was determined at 500nm and 570nm, respectively, by adding 0.021ka-2-naphthoquinone-4-sulfonate sodium 1% hexadecyltrimethylammonium bromide and 2sodium hydroxide (or 3Na2CO3) (1: 6: 1: 2 or 3). The absorptivity was determined at 500nm and 570nm, respectively. The resolution and repeatability of determination of phenacetin and paracetamol in plasma samples by this technique are comparable to those of HPLC. Conclusion the content of phenacetin and paracetamol in blood was determined by spectrophotometry. The ratio of phenacetin and paracetamol has the value of evaluating liver reserve function.
【作者单位】：山东省千佛山医院;
【基金】：山东省科技攻关项目(2009GG10002005) 山东省医药卫生科研项目(2007HW116)
【分类号】：R575

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