父母单一感染患者辅助胚胎HBV DNA检测对乙型肝炎病毒感染的诊断价值研究

发布时间：2018-07-06 13:41

本文选题：乙型肝炎病毒 + 感染　；参考：《中华医院感染学杂志》2017年21期

【摘要】：目的探讨乙型肝炎病毒(HBV)父母单一感染者辅助生殖助孕废弃胚胎中HBV DNA检测结果及影响因素,为HBV垂直传播的预防提供参考依据。方法选取2010年1月-2016年12月医院行辅助生殖助孕的200对夫妇废弃的胚胎200个,采用套式聚合酶链反应(PCR)对HBV DNA进行检测,以时间分辨荧光免疫分析技术检测父母感染者血清乙肝七项、荧光定量PCR法检测HBVDNA载量。结果 HBV单一感染父母HBsAg与HBcAb均为阳性,阳性率为100.00%,HBsAb、HBeAg、HBeAb、HBcAb-IgM、HBs1Ag阳性者分别有0例、59例、94例、7例、138例,阳性率为0.00%、29.50%、47.00%、3.50%、69.00%,HBVDNA阳性者200例,阳性率为100.00%,HBV DNA高载量者52例,阳性率为26.00%;200个胚胎,其中17个胚胎检测显示HBV DNA阳性,阳性率为8.50%;单因素分析,男方与女方年龄、受精方式、辅助生殖助孕次数、HBV感染者性别、HBs1Ag检测结果对胚胎HBV DNA检测结果无影响,HBeAg阳性、HBeAb阳性、HBcAb-IgM阳性、HBV DNA高载量阳性时胚胎HBV DNA阳性率分别为15.25%、13.83%、42.86%、19.23%,阴性时胚胎HBV DNA阳性率分别为5.67%、3.77%、7.25%、4.73%,比较差异有统计学意义(P0.05),结果显示HBeAg、HBeAb、HBcAb-IgM、HBV DNA高载量检测结果对胚胎HBV DNA检测结果存在影响;多因素logistic回归分析结果显示HBV DNA高载量是胚胎HBV DNA检测结果的独立影响因素(P0.05)。结论 HBV父母单一感染可引起胚胎HBV DNA阳性,感染者HBV DNA高载量是胚胎HBV感染的高风险因素,建议在受孕前应进行针对性治疗,以降低胚胎感染HBV风险。
[Abstract]:Objective to investigate the results of HBV DNA detection and its influencing factors in assisted reproductive assisted pregnancy abandoned embryos from single infected parents of hepatitis B virus (HBV) parents, and to provide a reference for the prevention of HBV vertical transmission. Methods from January 2010 to December 2016, 200 abandoned embryos were collected from 200 couples who received assisted reproductive assistance in hospitals. HBV DNA was detected by nested polymerase chain reaction (PCR), and HBV DNA was detected by nested polymerase chain reaction (PCR), and HBV DNA was detected by nested polymerase chain reaction (PCR). Seven items of hepatitis B were detected by time-resolved fluorescence immunoassay and HBV DNA load was detected by fluorescence quantitative PCR. Results both HBsAg and HBcAb were positive in parents with HBV single infection, and the positive rate was 100.00HBeAgHBeAb-HBcAb-IgMHB1Ag positive. There were 0 cases of 59 cases with HBcAb-IgMHB1Ag positive, respectively. The positive rate was 138 cases. The positive rate was 0.0047.003.50 + HBVDNA positive in 200 cases, and the positive rate was 100.00000 in 52 cases with high HBV DNA load, the positive rate was 26.00; 200 embryos, 200 embryos, Among them, 17 embryos were positive for HBV DNA, the positive rate was 8.50.The univariate analysis showed that the age of male and female, the way of fertilization, The positive rate of HBV-DNA in embryos with HBeAg-positive HBeAg-positive HBeAb + HBeAb + HBeAb and HBV-DNA was 15.2535% 13.833.35% and 19.23% respectively when the number of assisted reproductive aids was higher than that of HBV-infected persons. The positive rate of HBV DNA in the embryos was 19.23% in negative cases, and the positive rate of HBV-DNA in the embryos was higher than that in the cases of HBeAg + HBeAb + HBeAb + HBeAb + HBeAb + HBeAb + HBeAb. The difference was statistically significant (P 0.05). The results showed that the high load of HBcAb-IgM DNA in HBeAgN HBeAg + HBeAb-HBcAb-IgMU had an effect on the results of HBV DNA detection in embryos. Multivariate logistic regression analysis showed that the high load of HBV-DNA was an independent factor in the detection of HBV DNA in embryos (P0.05). Conclusion single infection of HBV parents can cause HBV DNA positive in embryos. The high load of HBV DNA in infected patients is a high risk factor for HBV infection in embryos. It is suggested that targeted treatment should be carried out before conception in order to reduce the risk of HBV infection in embryos.
【作者单位】：遵义医学院附属医院生殖中心;
【分类号】：R512.62

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