NF-κB信号通路在小鼠阻塞性黄疸及运动与黑果枸杞多糖干预中的差异表达

发布时间：2018-07-09 19:05

本文选题：黑果枸杞多糖 + 有氧运动　；参考：《中国体育科技》2017年04期

【摘要】：目的:探讨NF-κB信号通路在小鼠阻塞性黄疸致肝损伤的机理及阐述运动联合黑果枸杞多糖改善肝损伤的效果。方法:KM小鼠随机分为5组:假手术组、模型组、枸杞组、运动组、枸杞+运动组。应用HE染色、免疫组化法、Realtime-PCR技术对肝组织进行检测。结果:1)HE染色显示,假手术组肝细胞索排列整齐;模型组大鼠肝细胞纤维化,可见片状肝细胞变性、坏死,乃至肝细胞索结构紊乱;枸杞组、运动组可见肝细胞少量点、片状坏死,肝小叶结构改变,肝索排列欠整齐;枸杞+运动组无明显组织坏死,肝小叶结构基本正常,肝索排列较整齐。免疫组织化学染色结果显示,枸杞+运动组小鼠肝组织NF-KB P65、TNF-α阳性表达均显著低于模型组。Real-time PCR结果显示,与模型组比较,枸杞+运动组小鼠血清NF-k B、TNF-α、TGF-β、IL-6 m RNA表达降低。结论:运动联合黑果枸杞多糖能改善肝损伤小鼠的免疫功能,对阻塞性黄疸小鼠的肝细胞具有修复作用。
[Abstract]:Aim: to investigate the mechanism of NF- 魏 B signaling pathway in liver injury induced by obstructive jaundice in mice and to elucidate the effect of exercise combined with Lycium barbarum polysaccharides on liver injury. Methods Twenty km mice were randomly divided into 5 groups: sham operation group, model group, medlar group, exercise group and medlar exercise group. Liver tissue was detected by HE staining and Realtime PCR. Results (1) he staining showed that the hepatic cells in sham-operated group were arranged neatly; in model group, hepatocyte fibrosis, degeneration, necrosis, and even disorder of hepatic cell cord were observed; in Lycium barbarum group, a small number of hepatocytes were found in exercise group. In Lycium barbarum exercise group, there was no obvious tissue necrosis, the structure of hepatic lobule was normal, and the arrangement of hepatic cord was neat. The results of immunohistochemical staining showed that the positive expression of NF-KB P65 TNF- 伪 in liver tissue of Lycium barbarum exercise group was significantly lower than that of model group. Real-time PCR showed that the expression of IL-6 mRNA in serum of Lycium barbarum exercise group was lower than that in model group. Conclusion: exercise combined with Lycium barbarum polysaccharides can improve the immune function of mice with liver injury and repair the hepatocytes of obstructive jaundice mice.
【作者单位】：湖南师范大学体育学院;湖南中医药高等专科学校;邵阳市中心医院;宜春学院;
【分类号】：R575

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