功能性消化不良患者唾液菌群的研究

发布时间：2018-07-26 07:55

【摘要】：目的分析功能性消化不良患者与健康对照者唾液菌群的差异。方法收集功能性消化不良患者及健康对照者的唾液标本,提取总基因组DNA,采用高通量测序技术对样本中细菌的16SrRNA-V4区进行DNA测序后进行生物信息学分析。结果功能性消化不良组唾液中的菌群以变形菌门为主,健康对照组以拟杆菌门为主。功能性消化不良唾液菌群的Chao1、ACE、Shannon指数分别为1 295、1 351、4.93,健康对照组为1 001、1 019、5.28。PCoA可基本将两组间的唾液菌群区分开。LEfSe分析发现两组间有差异菌群共有16个,包括普式菌属、奈瑟菌属及变形菌门等。结论功能性消化不良患者唾液有其特征性的菌群组成,以变形菌门为主,但两组间的丰富度及多样性无差别,奈瑟菌属是两组间差异显著的细菌之一。
[Abstract]:Objective to analyze the differences of salivary flora between patients with functional dyspepsia and healthy controls. Methods the total genomic DNA was extracted from saliva samples of patients with functional dyspepsia and healthy controls. The 16SrRNA-V4 region of bacteria was sequenced by high-throughput sequencing technique and bioinformatics analysis was carried out. Results the bacteria in saliva of functional dyspepsia group were mainly Proteus, while that of healthy control group was Bacteroid phylum. The Shannon index of Chao1-ACESe in functional dyspepsia saliva was 1 295 / 1 351N 4.93, respectively, and that in the healthy control group was 1 001 / 1 019 / 5.28.PCoA, which could basically distinguish the salivary flora of the two groups. LEfSe analysis showed that there were 16 different microflora in the two groups, including the genus Pu. Neisseria and Proteus, etc. Conclusion saliva of patients with functional dyspepsia has its own characteristic flora composition, mainly Proteus, but there is no difference in richness and diversity between the two groups. Neisseria is one of the bacteria with significant difference between the two groups.
【作者单位】：南方医科大学;广东省人民医院/广东省医学科学院;
【基金】：广东省省级科技计划项目(2013B021800198)
【分类号】：R57

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