维生素C和维生素D水平对溃疡性结肠炎患者炎症程度的影响

发布时间：2018-08-20 16:44

【摘要】：目的探讨溃疡性结肠炎(ulcerative colitis,UC)病人体内维生素C(vitamin C,VC)和维生素D(vitamin D,VD)水平对炎症程度的影响。方法收集2016年1月至2016年12月在山西省大同市同煤集团总医院确诊的40例缓解期UC患者,采用高效液相色谱(high performance liquid chromatography,HPLC)紫外检测法检测血清中VC水平,根据VC水平将其分为高VC组(2~4mg/L)和低VC组(2mg/L)。采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)和比色法检测血清中25-(OH)D_3、1,25-(OH)_2D_3、TNF-α、IL-6、IL-1β和髓过氧化物酶(myeloperoxidase,MPO)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的水平,比较两组间的差异。结果高VC组25-(OH)D_3、1,25-(OH)_2D_3水平高于低VC组,高VC组25-(OH)D_3与低VC组有统计学差异(P0.05)。高VC组血清中TNF-α、IL-6、IL-1β、MPO水平低于低VC组,差异均有统计学意义(P0.05)。高VC组血清GSH-Px高于低VC组,尚无统计学差异。结论 UC患者血清VC水平影响25-(OH)D_3和1,25-(OH)_2D_3的水平,表明VC可能参与VD的活化,在适宜范围内VC与25-(OH)D_3、1,25-(OH)_2D_3呈正相关,1,25-(OH)_2D_3通过免疫机制参与UC的发病和发展,与UC的严重程度呈负相关。
[Abstract]:Objective to investigate the effect of vitamin C (vitamin C) and vitamin D (vitamin D) on inflammation in patients with ulcerative colitis (ulcerative colitis). Methods from January 2016 to December 2016, 40 patients with remission UC who were diagnosed in Datong Coal Group General Hospital of Datong City, Shanxi Province were collected. The serum VC levels were detected by (high performance liquid chromatography-HPLC. According to VC level, it was divided into high VC group (2~4mg/L) and low VC group (2mg/L). Enzyme linked immunosorbent assay (Elisa) and colorimetric assay (Elisa) were used to detect the levels of serum 25- (OH) DX, 2DNF- 伪 TNF- 伪 TNF- 伪, IL-6, IL-1 尾 and glutathione peroxidase (glutathione peroxidase GSH-Px) in serum, and the differences between the two groups were compared. Results the levels of 25- (OH) D3 and 25- (OH) in high VC group were higher than those in low VC group. There was a significant difference between high VC group and low VC group in 25 (OH) D3 (P0.05). The serum levels of TNF- 伪 IL-6 and IL-1 尾 MPO in high VC group were significantly lower than those in low VC group (P0.05). The serum GSH-Px in high VC group was higher than that in low VC group, but there was no statistical difference. Conclusion the level of VC in serum of UC patients affects the levels of 25- (OH) D3 and 1- 25- (OH) 2DSP, which indicates that VC may be involved in the activation of VD. Within the appropriate range, VC and 25- (OH) D3D3 have a positive correlation with (OH) 2D3. They are involved in the pathogenesis and development of UC through the immune mechanism, and are negatively correlated with the severity of UC.
【作者单位】：山西医科大学公共卫生学院营养与食品卫生教研室;同煤集团总医院消化内科;山西医科大学第一附属医院消化内科;
【基金】：国家自然科学基金(No.81573156) 山西医科大学大学生创新创业校级项目(No.20160326)
【分类号】：R574.62

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