趋化因子Fractalkine及其受体CX3CR1预测重型急性胰腺炎的价值研究

发布时间：2018-08-26 10:27

【摘要】：目的检测重型急性胰腺炎(SAP)患者血浆中趋化因子Fractalkine(FKN)及其受体CX3CR1的表达水平,判断其能否作为SAP的预测指标。方法选取2012年12月—2014年9月宁夏医科大学总医院收治的因急性上腹痛起病,症状出现在3 d以内,依据2013年《中国急性胰腺炎诊治指南》诊断为急性胰腺炎的患者65例为研究对象,其中轻型急性胰腺炎(MAP)20例(30.8%),中型急性胰腺炎(MSAP)25例(38.4%),SAP 20例(30.8%),应用酶联免疫吸附试验(ELISA)法检测患者血浆中FKN及其受体CX3CR1表达水平。比较不同病情程度急性胰腺炎患者FKN及其受体CX3CR1表达水平,绘制受试者工作特征曲线(ROC曲线),计算ROC曲线下面积(AUC),判定预测SAP的最佳截点及其灵敏度、特异度、正确率。结果不同病情程度急性胰腺炎患者FKN及其受体CX3CR1表达水平比较,差异均有统计学意义(P0.05);其中SAP患者FKN及其受体CX3CR1表达水平高于MAP、MSAP患者(P0.05)。FKN及其受体CX3CR1预测SAP的AUC分别为0.865、0.897,最佳截点分别为2.08μg/L和38μg/L,灵敏度分别为86.96%、90.91%,特异度分别为76.27%、88.24%,正确率分别为79.27%、89.04%。结论SAP患者FKN及其受体CX3CR1表达水平高于MAP、MSAP患者,FKN及其受体CX3CR1可作为预测急性胰腺炎严重程度的有效指标之一,值得进一步深入研究。
[Abstract]:Objective to detect the expression of chemokine Fractalkine (FKN) and its receptor CX3CR1 in plasma of patients with severe acute pancreatitis (SAP) and to determine whether they can be used as predictors of SAP. Methods from December 2012 to September 2014, patients with acute epigastric pain were admitted to the General Hospital of Ningxia Medical University, and the symptoms appeared within 3 days. According to the Chinese guidelines for the diagnosis and treatment of Acute pancreatitis in 2013, 65 patients with acute pancreatitis were studied. There were 20 cases (30.8%) with mild acute pancreatitis and 25 cases (38.4%) with (MSAP) with moderate acute pancreatitis. The expression of FKN and its receptor CX3CR1 in the plasma of patients with mild acute pancreatitis and moderate acute pancreatitis were detected by enzyme-linked immunosorbent assay (ELISA). The expression levels of FKN and its receptor CX3CR1 in patients with acute pancreatitis with different severity were compared, and the operating characteristic curve (ROC curve) was drawn. The area (AUC), under the ROC curve was calculated to determine the best cut-off point, sensitivity, specificity and accuracy of SAP prediction. Results the expression levels of FKN and its receptor CX3CR1 in patients with acute pancreatitis with different severity were compared. The expression level of FKN and its receptor CX3CR1 in SAP patients was significantly higher than that in MAP,MSAP patients (P0.05). FKN and its receptor CX3CR1 were used to predict the AUC of SAP were 0.865 渭 g / L and 38 渭 g / L, respectively, and the sensitivity was 86.96g / L, the specificity was 76.2788.248.24, and the correct rate was 79.270.89.04. Conclusion the expression of FKN and its receptor CX3CR1 in SAP patients is higher than that in MAP,MSAP patients. It can be used as an effective index to predict the severity of acute pancreatitis.
【作者单位】：宁夏医科大学;宁夏医科大学总医院消化内科;
【基金】：国家自然科学基金资助项目(81260086)
【分类号】：R576

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