雌激素对去势大鼠肝脂肪变性影响及炎性机制

发布时间：2018-09-05 10:56

【摘要】：目的观察雌激素对去势大鼠肝脂肪变作用,探讨雌激素肝保护作用及可能机制。方法 6周SD雌性大鼠随机分为3组:假手术组、手术组和雌二醇组,术后1周,雌二醇组大鼠每天灌胃戊酸雌二醇(1 mg/kg)水溶液,其他组大鼠给予蒸馏水,连续12周。进行胰岛素耐量试验,采用酶联免疫法测定肝组织中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)和单核细胞趋化蛋白-1(MCP-1)含量。苏木素-伊红染色观察肝脂肪变性和炎症状况。结果与假手术组比较,手术组大鼠体重、肝重、内脏脂肪重量[分别为(439.9±53.1)、(12.6±1.9)、(29.6±12.1)g]及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平[分别为(57.8±5.4)、(128.4±10.1)U/L]明显升高(P0.05);与手术组比较,雌二醇组大鼠体重、肝重、内脏脂肪重量[分别为(387.3±40.7)、(10.3±2.1)、(24.1±6.5)g]及血清ALT、AST水平[分别为(44.5±4.8)、(98.2±9.6)U/L]明显降低(P0.05)。手术组大鼠肝小叶结构模糊,肝索排列紊乱,有广泛的脂滴形成,小叶内及汇管区可见炎症细胞浸润;雌二醇组大鼠肝小叶结构仍欠清晰,脂滴明显减少,且脂滴明显变小;与手术组比较,雌二醇组大鼠肝脂肪变程度及小叶内炎症程度显著减轻。与假手术组比较,手术组大鼠肝组织匀浆中TNF-α、IL-6、MCP-1含量[分别为(186.3±17.2)、(187.6±26.5)、(112.3±22.4)pg/m L]明显升高(P0.05);与手术组比较,雌二醇组大鼠肝组织匀浆中TNF-α、IL-6、MCP-1含量[分别为(132.6±8.6)、(110.8±21.2)、(89.1±10.8)pg/m L]明显降低(P0.05)。结论雌激素可减轻去势大鼠肝脂肪变性程度,其机制可能与降低肝组织炎性因子表达有关。
[Abstract]:Objective to observe the effect of estrogen on hepatic adipogenesis in ovariectomized rats and to explore the protective effect and possible mechanism of estrogen on liver. Methods six weeks SD female rats were randomly divided into three groups: sham operation group, operation group and estradiol group. One week after operation, estradiol group rats were gavaged with estradiol valerate (1 mg/kg) solution daily, and other rats were given distilled water for 12 weeks. The contents of tumor necrosis factor- 伪 (TNF- 伪), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) in liver tissue were measured by enzyme linked immunosorbent assay (Elisa). Hepatic steatosis and inflammation were observed by hematoxylin-eosin staining. Results compared with sham operation group, the body weight, liver weight, visceral fat weight [(439.9 卤53.1), (12.6 卤1.9), (29.6 卤12.1 g) g] and serum alanine aminotransferase (ALT),) transaminase (AST) levels [(57.8 卤5.4), (128.4 卤10.10) U / L] in the operation group were significantly higher than those in the operation group (P0.05), and the weight of the rats in the estradiol group was significantly higher than that in the operation group (P0.05). Liver weight, visceral fat weight (387.3 卤40.7), (10.3 卤2.1), (24.1 卤6.5g) and serum ALT,AST level (44.5 卤4.8), (98.2 卤9.6U / L) were significantly decreased (P0.05). In the operation group, the hepatic lobule structure was blurred, the hepatic cord arranged disorderly, the lipid droplets were formed extensively, inflammatory cells were infiltrated in the lobules and in the catchment area, the structure of the hepatic lobules in the estradiol group was still unclear, the lipid droplets were obviously decreased, and the lipid droplets were obviously decreased. Compared with the operation group, the degree of hepatic fatty degeneration and intralobular inflammation in the estradiol group was significantly reduced. Compared with the sham operation group, the content of TNF- 伪 -IL-6 MCP-1 in the liver homogenate of the operation group [(186.3 卤17.2), (187.6 卤26.5), (112.3 卤22.4 pg/m L, respectively)] was significantly higher (P0.05), and the content of TNF- 伪 -IL-6MCP-1 in the liver tissue homogenate of the Estradiol group was significantly lower than that in the control group [(132.6 卤8.6), (110.8 卤21.2), (89.1 卤10.8) pg/m L] (P0.05). Conclusion estrogen can reduce the degree of hepatic steatosis in ovariectomized rats, and its mechanism may be related to the decrease of inflammatory factor expression in liver tissue.
【作者单位】： 淄博市妇幼保健院;中南大学代谢内分泌研究所;
【分类号】：R575.5
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本文编号：2224059