HBV感染者脂肪间充质干细胞体外诱导为肝样细胞

发布时间：2018-09-05 11:50

【摘要】：背景:有报道显示,人脂肪间充质干细胞在体外特定为环境下可诱导分化为具有白蛋白合成功能及分泌尿素功能的肝样细胞。目的:探讨乙型肝炎病毒感染者皮下脂肪组织间充质干细胞体外向肝样细胞分化的潜能。方法:胶原酶消化乙型肝炎病毒感染者脂肪组织,结合贴壁培养法获得脂肪间充质干细胞。采用三阶段诱导法诱导脂肪间充质干细胞向肝样细胞分化,观察其形态变化,免疫组织化学染色检测肝细胞相关蛋白甲胎蛋白、白蛋白和细胞角蛋白18表达,糖原染色法检测细胞糖原合成功能。结果与结论:脂肪间充质干细胞经3阶段诱导培养后,大部分细胞成多角形,类肝细胞形态;免疫组织化学染色结果显示,诱导培养后脂肪间充质干细胞白蛋白、细胞角蛋白18和甲胎蛋白染色阳性,且细胞白蛋白和细胞角蛋白18的表达随着诱导培养时间的延长逐渐增加;诱导培养后的脂肪间充质干细胞具有糖原合成功能,过碘酸雪夫试剂染色阳性。结果表明,乙型肝炎病毒感染者皮下脂肪组织间充质干细胞经成肝诱导培养后可分化为有功能的肝样细胞。
[Abstract]:Background: it has been reported that human adipose mesenchymal stem cells can be induced to differentiate into hepatoid cells with albumin synthesis and urea secretion in vitro. Aim: to investigate the differentiation potential of subcutaneous adipose mesenchymal stem cells (MSCs) from hepatitis B virus infected patients into hepatocytes in vitro. Methods: adipose tissue of hepatitis B virus infected patients was digested by collagenase, and adipose mesenchymal stem cells were obtained by adherent culture. The differentiation of adipose mesenchymal stem cells into hepatocyte-like cells was induced by three-stage induction. The expression of hepatocyte associated protein alpha-fetoprotein, albumin and cytokeratin 18 was detected by immunohistochemical staining. Glycogen synthesis was detected by glycogen staining. Results and conclusion: after three stages of induction and culture, most of the adipose mesenchymal stem cells were polygonal and hepatocyte-like, and the results of immunohistochemical staining showed that the adipose mesenchymal stem cells had albumin in the cultured adipose mesenchymal stem cells. Cytokeratin 18 and alpha-fetoprotein were positive, and the expression of albumin and cytokeratin 18 gradually increased with the prolongation of culture time, and adipose mesenchymal stem cells had glycogen synthesis function. Scheffer's reagent of periodate was positive. The results showed that mesenchymal stem cells of subcutaneous adipose tissue induced by hepatitis B virus could differentiate into functional hepatocytes.
【作者单位】：吉林省肝胆病医院;舒兰市人民医院;
【分类号】：R512.62

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