感染后肠易激综合征小鼠不同肠段肠黏膜热休克蛋白70的表达及意义

发布时间：2018-09-14 20:41

【摘要】：目的探讨感染后肠易激综合征(PI-IBS)小鼠不同肠段肠黏膜热休克蛋白70(HSP70)的表达,及其与炎症细胞因子表达的相关性。方法旋毛虫感染小鼠,不同肠段肠黏膜组织苏木精-伊红染色法(HE)染色,炎症评分。腹壁撤退反射(AWR)和结肠传输试验(CTT)观察内脏敏感性和肠道动力变化。免疫组织化学及酶联免疫吸附试验(ELISA)检测不同肠段HSP70和炎症细胞因子的表达。Spearman相关性分析HSP70与炎症细胞因子表达的相关性。结果 1小鼠感染旋毛虫后末段回肠和结肠出现明显炎症,炎症消退后仍存在明显的内脏高敏感和肠道动力异常。2PI-IBS小鼠空肠和末段回肠的HSP70表达较对照组明显增强(P0.01)。3PI-IBS小鼠末段回肠白细胞介素-17(IL-17)、干扰素-γ(IFN-γ)水平明显升高,空肠、末段回肠及结肠白细胞介素-10(IL-10)水平明显降低(P0.01)。4PI-IBS小鼠空肠HSP70表达与炎症细胞因子IL-10呈负相关(P0.01);末段回肠HSP70表达与IL-10呈负相关(P0.01),与IL-17、IFN-γ呈正相关(P0.01)。结论 1不同肠段HSP70和炎症细胞因子的相关差异性表达可能在PI-IBS发病中起作用。2末段回肠可能是PI-IBS最重要的炎症免疫启动和调控部位。
[Abstract]:Objective to investigate the expression of heat shock protein 70 (HSP70) in different intestinal segments of irritable bowel syndrome (PI-IBS) mice and its correlation with inflammatory cytokines. Methods the intestinal mucosa of mice infected with Trichinella spiralis was stained by hematoxylin-eosin staining and inflammatory score. Abdominal withdrawal reflex (AWR) and colonic transit test (CTT) were used to observe the visceral sensitivity and intestinal motility. Immunohistochemistry and enzyme linked immunosorbent assay (ELISA) were used to detect the expression of HSP70 and inflammatory cytokines in different intestinal segments. The correlation between HSP70 and inflammatory cytokines was analyzed. Results 1the ileum and colon of mice infected with Trichinella spiralis showed obvious inflammation. The expression of HSP70 in jejunum and ileum of mice with abnormal intestinal motility and visceral hypersensitivity after inflammation subsided was significantly higher than that in control group (P0.01) .3The level of Interferon- 纬 (IFN- 纬) and jejunum were significantly increased compared with the control group (P0.01). The levels of interleukin-10 (IL-10) in the ileum and colon decreased significantly (P0.01) .4The expression of HSP70 in jejunum was negatively correlated with the inflammatory cytokine IL-10 (P0.01), and the expression of HSP70 in the terminal ileum was negatively correlated with IL-10 (P0.01) and positively correlated with IL-17,IFN- 纬 (P0.01). Conclusion 1 the differential expression of HSP70 and inflammatory cytokines in different intestinal segments may play an important role in the pathogenesis of PI-IBS. 2. The ileum may be the most important inflammatory immune priming and regulating site of PI-IBS.
【作者单位】：海南省人民医院消化内科;重庆医科大学附属第一医院消化内科;
【基金】：国家自然科学基金(No:81160057) 海南省国际科技合作专项基金(No:KJHZ2013-14)
【分类号】：R574.4

【参考文献】