双歧杆菌对高脂饮食诱导非酒精性脂肪性肝病大鼠血脂代谢的影响

发布时间：2018-10-11 15:49

【摘要】：目的探讨双歧杆菌对高脂饮食诱导非酒精性脂肪性肝病大鼠血脂代谢的影响。方法将45只雄性SD大鼠随机等分为对照组、模型组和治疗组。对照组喂以普通饲料,模型组喂以高脂饲料,治疗组喂以高脂饲料和双歧杆菌。6 w后比较各组大鼠肝指数和肝组织学改变,检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆固醇(TC)和甘油三酯(TG)的水平以及肝总脂肪(HLs)含量,采用荧光实时定量RT-PCR测定禁食诱导脂肪因子(FIAF)mRNA的表达。结果模型组肝指数和肝组织脂肪变性程度均显著高于对照组(P0.05);而治疗组肝指数和肝组织脂肪变性程度均显著低于模型组(P0.05)。与对照组比较,模型组ALT、AST、TC、TG和HLs水平显著升高(P0.05);与模型组比较,治疗组ALT、AST、TC、TG和HLs水平显著降低(P0.05)。与对照组比较,模型组FIAF mRNA的表达显著升高(P0.05);与模型组比较,治疗组FIAF mRNA的表达显著降低(P0.05)。结论双歧杆菌能够降低NAFLD大鼠的血脂水平,减少肝脏组织中脂肪的沉积,改善肝脏的炎症反应,其机制可能与调节脂质的摄入和抑制FIAF基因的表达有关。
[Abstract]:Objective to investigate the effect of Bifidobacterium on lipid metabolism in rats with non-alcoholic fatty liver disease induced by high fat diet. Methods 45 male SD rats were randomly divided into control group, model group and treatment group. The control group was fed with normal diet, the model group with high fat diet, the treatment group with high fat diet and Bifidobacterium. After 6 weeks, the liver index and liver histology of rats in each group were compared. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG) and hepatic total fat (HLs) were measured. The expression of adipose factor (FIAF) mRNA induced by fasting was measured by real-time quantitative RT-PCR. Results the liver index and the degree of steatosis in the model group were significantly higher than those in the control group (P0.05), while the liver index and the degree of steatosis in the treatment group were significantly lower than those in the model group (P0.05). Compared with the control group, the levels of ALT,AST,TC,TG and HLs in the model group were significantly higher (P0.05), and the levels of ALT,AST,TC,TG and HLs in the treatment group were significantly lower than those in the model group (P0.05). Compared with the control group, the expression of FIAF mRNA in the model group was significantly increased (P0.05); compared with the model group, the expression of FIAF mRNA was significantly decreased in the treatment group (P0.05). Conclusion Bifidobacterium can decrease the level of serum lipids, decrease the fat deposition in the liver and improve the inflammatory response of the liver in NAFLD rats. The mechanism may be related to the regulation of lipid intake and the inhibition of the expression of FIAF gene.
【作者单位】：华中科技大学同济医学院附属普爱医院消化内科;
【基金】：武汉市卫生局临床科研资助项目(WX12C33)
【分类号】：R575.5

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