肠道缺血再灌注对小鼠体内脂肪细胞因子chemerin表达的影响

发布时间：2018-10-18 14:05

【摘要】：目的探讨小鼠肠道缺血再灌注损伤对体内组织器官脂肪细胞因子chemerin的影响。方法无特定病原体级C57BL/6J小鼠16只,体质量(25±2)g,适应性饲养5 d后,按照随机数字表尾数法分为肠道缺血再灌注组和假手术组,每组8只。肠道缺血再灌注组夹闭小鼠肠系膜上动脉60 min,然后再灌注60 min造成肠道损伤,假手术组只开腹不夹闭120 min。实验结束后,处死动物取材检测。应用酶联免疫吸附实验方法测定各组织器官chemerin的浓度变化,用Western blot方法检测chemerin蛋白表达。结果酶联免疫吸附实验结果显示肠道缺血再灌注损伤后小鼠体内各组织器官chemerin浓度显著增加(P0.05),Western blot结果显示chemerin蛋白表达显著增加(P0.05)。结论肠道缺血再灌注损伤时小鼠各器官组织的脂肪细胞因子chemerin浓度和蛋白表达显著增加。
[Abstract]:Objective to investigate the effect of intestinal ischemia reperfusion injury on adipocyte factor chemerin in tissues and organs of mice. Methods Sixteen C57BL/6J mice (25 卤2 g) were randomly divided into intestinal ischemia-reperfusion group and sham-operation group according to the random number Mantissa method after 5 days of adaptive feeding. 8 mice in each group were divided into two groups: intestinal ischemia reperfusion group (n = 8) and sham operation group (n = 8). Intestinal injury was induced by occlusion of superior mesenteric artery for 60 min, and reperfusion for 60 min in the intestinal ischemia-reperfusion group, and no clamping was performed in the sham operation group for 120 min.. At the end of the experiment, the animals were sacrificed for examination. Enzyme linked immunosorbent assay (Elisa) was used to detect the concentration of chemerin in tissues and organs, and chemerin protein expression was detected by Western blot method. Results the results of enzyme-linked immunosorbent assay (Elisa) showed that the concentration of chemerin in tissues and organs of mice after intestinal ischemia-reperfusion injury was significantly increased (P0.05), Western blot results showed a significant increase in the expression of chemerin protein (P0.05). Conclusion the concentration of adipocyte factor chemerin and the expression of protein in various organs of mice were significantly increased during intestinal ischemia reperfusion injury.
【作者单位】：中国医学科学院北京协和医学院北京协和医院肠外肠内营养科;
【基金】：国家自然科学基金(30940069)~~
【分类号】：R574

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