肝过表达hNPC1L1转基因巴马小型猪的初步研究

发布时间：2018-10-23 16:47

【摘要】：研究利用体细胞核移植技术构建了肝过表达h NPC1L1转基因巴马小型猪,由PCR和RT-PCR鉴定表明h NPC1L1在肝组织中特异性地表达。我们对转基因巴马小型猪进行了初步研究,对血清和肝匀浆中的TC、TG、LDL-C、HDL-C、AST、ALT和FFA进行了检测,结果表明转基因猪血清FFA(637.7±9.53)μmol/L显著高于非转基因猪血清FFA(540.4±25.46)μmol/L(p0.01),转基因猪血清AST(200.8±22.31)U/L显著高于非转基因猪血清AST(92.4±15.55)U/L(p0.01)。另外,我们对肝中与脂代谢密切相关的基因表达情况进行Real-time PCR检测的结果表明,转基因猪SREBP-1c(3.31±0.18,p0.05)、FAS(4.49±4.21,p0.01)显著高于非转基因猪。本研究发现NPC1L1在与脂代谢紊乱密切相关的肝脏疾病中发挥着主导作用,它能通过对脂合成相关基因转录的调控,引发脂代谢紊乱以及肝脏疾病。这说明对NPC1L1的研究有望成为治疗肝脏疾病的新靶标。
[Abstract]:In this study, we used somatic cell nuclear transfer technique to construct h NPC1L1 transgenic miniature pig. PCR and RT-PCR analysis showed that h NPC1L1 was specifically expressed in liver tissue. TC,TG,LDL-C,HDL-C,AST,ALT and FFA in serum and liver homogenate were detected. The results showed that the FFA of transgenic pig serum (637.7 卤9.53) 渭 mol/L was significantly higher than that of non-transgenic pig serum FFA (540.4 卤25.46) 渭 mol/L (p0.01), and that of transgenic pig serum AST was (200.8 卤22.31) U / L significantly higher than that of non-transgenic pig serum AST (92.4 卤15.55) U / L (p0.01). In addition, the expression of genes closely related to lipid metabolism in the liver was detected by Real-time PCR. The results showed that the SREBP-1c of transgenic pigs (3.31 卤0.18p0.05), FAS (4.49 卤4.21), FAS (p0.01) was significantly higher than that of non-transgenic pigs. In this study, we found that NPC1L1 plays a leading role in liver diseases closely related to lipid metabolism disorder, which can induce lipid metabolism disorders and liver diseases by regulating the transcription of lipid biosynthesis related genes. This suggests that the study of NPC1L1 is expected to be a new target for the treatment of liver diseases.
【作者单位】：吉林化工学院生物与食品工程学院;韶关学院英东生命科学院粤北生猪生产及疫病防控协同创新发展中心;吉林农业大学动物科学技术学院;吉林大学动物医学学院;
【基金】：国家科技支撑计划(2011BA115B02)资助
【分类号】：R-332;R575

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