急性低氧对大鼠胃黏膜组织细胞端粒长度的影响及其机制
发布时间：2018-10-24 06:53
【摘要】：本文旨在观察急性低氧对大鼠胃黏膜组织细胞端粒长度的影响,并探讨其机制。40只雄性Wistar大鼠,随机分为对照组(兰州,1 500 m,n=10)与实验组(低压氧舱,5 000 m),实验组根据低氧暴露时间不同,又分为低氧1、3、7 d三组,每组各10只。低氧暴露完毕,取各组大鼠胃组织,行HE染色观察胃黏膜组织显微形态变化;采用real-time PCR法测胃黏膜组织细胞端粒长度;采用real-time PCR及ELISA法分别检测端粒酶逆转录酶(telomerase reverse transcriptase,TERT)、低氧诱导因子1α(hypoxia-inducible factor 1α,HIF-1α)、HIF-2α mRNA及蛋白表达水平;采用化学荧光法检测其活性氧(reactive oxygen species,ROS)水平;并分析TERT表达水平与HIF-1α、HIF-2α表达水平及端粒长度的相关性。结果显示,随着低氧时间的延长,胃黏膜组织细胞损伤逐渐加重,ROS生成量逐渐升高,端粒长度逐渐增加,TERT和HIF-1α mRNA及蛋白表达水平的变化趋势与端粒长度的变化趋势一致,而HIF-2α表达水平未见明显变化,仅在低氧7 d时表达增加;TERT mRNA表达水平与端粒长度之间、TERT mRNA和蛋白表达水平与HIF-1α之间均存在正相关性,而TERT mRNA表达水平与HIF-2α之间无明显相关性。上述结果提示,急性高原严重低氧环境下,机体产生大量ROS,损伤胃黏膜组织细胞,同时又通过HIF-1α上调TERT的表达及端粒酶活性,增加细胞端粒长度,使胃黏膜组织细胞产生适应性反应,避免发生严重损伤。
[Abstract]:The aim of this study was to observe the effect of acute hypoxia on telomere length of gastric mucosa in rats and to explore its mechanism. The experimental group was randomly divided into three groups: control group (Lanzhou, 1 500 mNM) and experimental group (low pressure oxygen chamber, 5 000 m),). According to the time of hypoxia exposure, the experimental group was divided into 3 groups, 10 rats in each group. After hypoxic exposure, the gastric tissue of rats in each group was taken and observed by HE staining, and the telomere length of gastric mucosal cells was measured by real-time PCR method. The expression levels of telomerase reverse transcriptase (telomerase reverse transcriptase,TERT), hypoxia inducible factor 1 伪 (HIF-1 伪), HIF-2 伪 mRNA and protein were detected by real-time PCR and ELISA, respectively. The correlation between TERT expression and HIF-1 伪, HIF-2 伪 expression and telomere length was analyzed. The results showed that with the prolongation of hypoxic time, the injury of gastric mucosal tissue cells was gradually aggravated, the ROS production and telomere length were gradually increased, and the expression of TERT and HIF-1 伪 mRNA and protein were consistent with the changes of telomere length. However, there was no significant change in HIF-2 伪 expression level. Only at 7 days after hypoxia, there was a positive correlation between the expression level of, TERT mRNA and protein and the expression of, TERT mRNA and protein in telomere length, but there was no significant correlation between TERT mRNA expression level and HIF-2 伪 expression. These results suggest that in acute high altitude and severe hypoxia, a large amount of ROS, damages gastric mucosal cells, at the same time, HIF-1 伪 upregulated the expression of TERT and telomerase activity, and increased the telomere length of the cells. Make gastric mucosal tissue cells produce adaptive response to avoid serious damage.
【作者单位】： 青海省人民医院消化内科;
【基金】：supported by the Natural Science Youth Foundation of Qinghai Province,China(No.2015-ZJ-941Q)
【分类号】：R57
，
本文编号：2290641
[Abstract]:The aim of this study was to observe the effect of acute hypoxia on telomere length of gastric mucosa in rats and to explore its mechanism. The experimental group was randomly divided into three groups: control group (Lanzhou, 1 500 mNM) and experimental group (low pressure oxygen chamber, 5 000 m),). According to the time of hypoxia exposure, the experimental group was divided into 3 groups, 10 rats in each group. After hypoxic exposure, the gastric tissue of rats in each group was taken and observed by HE staining, and the telomere length of gastric mucosal cells was measured by real-time PCR method. The expression levels of telomerase reverse transcriptase (telomerase reverse transcriptase,TERT), hypoxia inducible factor 1 伪 (HIF-1 伪), HIF-2 伪 mRNA and protein were detected by real-time PCR and ELISA, respectively. The correlation between TERT expression and HIF-1 伪, HIF-2 伪 expression and telomere length was analyzed. The results showed that with the prolongation of hypoxic time, the injury of gastric mucosal tissue cells was gradually aggravated, the ROS production and telomere length were gradually increased, and the expression of TERT and HIF-1 伪 mRNA and protein were consistent with the changes of telomere length. However, there was no significant change in HIF-2 伪 expression level. Only at 7 days after hypoxia, there was a positive correlation between the expression level of, TERT mRNA and protein and the expression of, TERT mRNA and protein in telomere length, but there was no significant correlation between TERT mRNA expression level and HIF-2 伪 expression. These results suggest that in acute high altitude and severe hypoxia, a large amount of ROS, damages gastric mucosal cells, at the same time, HIF-1 伪 upregulated the expression of TERT and telomerase activity, and increased the telomere length of the cells. Make gastric mucosal tissue cells produce adaptive response to avoid serious damage.
【作者单位】： 青海省人民医院消化内科;
【基金】：supported by the Natural Science Youth Foundation of Qinghai Province,China(No.2015-ZJ-941Q)
【分类号】：R57
，
本文编号：2290641
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