福州地区献血者人群隐匿性乙型肝炎病毒感染及其基因型与S区突变的研究

发布时间：2018-11-04 16:25

【摘要】：目的探讨福州地区无偿献血者人群隐匿性乙型肝炎病毒感染(OBI)状况及其HBV基因型分布特征与S区氨基酸突变的情况。方法应用血清学方法与核酸检测技术(NAT),对福建省福州市2011年11月-2013年3月的102 866(人)份无偿献血者标本做常规HBs Ag筛查及HBV DNA检测,排除HBV血清学标志物乙肝两对半检测结果阴性标本,确定HBs Ag-HBV DNA+为OBI标本;应用实时荧光定量PCR技术对OBI标本做HBV DNA检测,S区基因采用巢式PCR扩增和序列测定,使用MEGA5.0软件对HBV基因分型和对S区氨基酸做突变分析。结果2011年11月-2013年3月福州地区无偿献血者标本共筛查出75例HBs Ag-HBV DNA+[0.073%(75/102 866)],其中OBI率0.064%(66/102 866);66例OBI标本中,抗-HBc阳性比例为93.94%(62/66),HBV DNA检出率18.18%(12/66),HBV DNA为(13-302)IU/m L,仅1例标本的HBV DNA200 IU/m L,15.15%(10/66)的OBI标本扩增出S区基因序列,其中B型7例、C型3例;突变分析发现在这10例中有7例HBV S区氨基酸发生突变,而其中又有6例的HBs Ag抗原决定簇基因及周边主要亲水区域(MHR)发生氨基酸突变。结论福州地区无偿献血者人群中存在一定的OBI感染率,其中抗-HBc阳性者比例占多数,OBI感染者的病毒载量低;HBV基因型主要以B型为主,HBV S区尤其是MHR的氨基酸突变可能是造成OBI发生的主要原因之一。
[Abstract]:Objective to investigate the (OBI) status of occult hepatitis B virus infection and the distribution of HBV genotypes and the amino acid mutation in S region of non-remunerated blood donors in Fuzhou area. Methods routine HBs Ag screening and HBV DNA detection were performed in 102 866 (human) blood donors from November 2011 to March 2013 in Fuzhou, Fujian Province, by serological method and nucleic acid detection technique (NAT),). The negative samples of HBV serological marker hepatitis B were excluded and HBs Ag-HBV DNA was confirmed as OBI. Real time fluorescence quantitative PCR was used to detect HBV DNA in OBI samples. Nested PCR amplification and sequencing were used to amplify S-region genes. HBV genotyping and S-region amino acid mutation were analyzed by MEGA5.0 software. Results from November 2011 to March 2013, 75 cases of HBs Ag-HBV DNA were screened out from free blood donors in Fuzhou area. The OBI rate was 0.064% (66 / 102,866). The positive rate of anti HBc was 93.94% (62 / 66), HBV DNA) in 66 OBI samples (18.18%) (12 / 66), HBV DNA was (13-302) IU/m L). The S region gene sequence was amplified from 15.15% (10 / 66) of OBI samples, including 7 cases of type B and 3 cases of type C. Mutation analysis revealed that 7 of the 10 cases had mutations in the S region of HBV, and 6 of them had mutations in the HBs Ag antigen determinant gene and (MHR) in the peripheral main hydrophilic region. Conclusion there is a certain OBI infection rate in the free blood donors in Fuzhou area, in which the proportion of anti-HBc positive is the majority, and the viral load of OBI infection is low. The amino acid mutation in, HBV S region, especially in MHR, may be one of the main causes of OBI in HBV genotype.
【作者单位】：福建省血液中心;福建医科大学基础医学院;
【基金】：福建省卫生厅青年科研课题(2012-1-13)
【分类号】：R446.1;R512.62

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