GnRH、SS及其受体的表达在颅脑火器伤下急性末端回肠炎中的变化

发布时间：2018-11-11 15:19

【摘要】：目的：研究实验犬在各种应激中促性腺激素释放激素（GnRH）、生长抑素(SS)浓度的变化及其受体在实验性急性末端回肠炎中的表达，初步探讨应激与激素及其受体的内在联系，揭示应激与肠道疾病的相关性。方法：选用健康成年雄性杂种犬40只，随机分成8组，每组5只：常温常湿正常血糖组（A组）、常温常湿高血糖组（G组）、高温高湿高血糖组（B组）、高温高湿正常血糖组（H组）、常温常湿正常血糖颅脑火器伤组（C组）、常温常湿高血糖颅脑火器伤组（D组）、高温高湿正常血糖颅脑火器伤组（E组）、高温高湿高血糖颅脑火器伤组（F组）。C、D、E、F组分别给予手枪弹颅脑火器伤造模。造模后立即将犬仰卧位固定于手术台上，并行气管插管、心电监护、心肺复苏等抢救措施，监测伤后心率、血压等生命体征；除心脏挤压及人工呼吸气囊挤压抢救外，，不做其他处理。在无菌条件下立即行腹部手术，剖腹寻找回盲部，切取1-3cm末端回肠组织立即离心保存，然后将残端吻合。根据时间梯度：0小时、0.5小时、1小时、1.5小时、2小时，各组实验犬每只采集外周血5个样本，立即离心保存。在此过程中尽量延缓实验犬生命，予以静脉补充液体。采用酶联免疫吸附试验(Enzyme-LinkedImmunosorbent Assay,ELISA)定量检测外周血中GnRH和SS的浓度变化；采用Westernblot检测末端回肠组织中GnRH受体、SS受体在A、B、F组中各时间点的表达。结果:1．术后各组实验犬血浆中GnRH浓度的变化：0h时8个实验组中各实验犬血浆中GnRH浓度差异不明显；在0.5h时各组实验犬血浆中GnRH浓度明显下降，考虑与其自身调节有关；随应激后时间延长GnRH浓度呈现递增趋势，且明显超过起始浓度（0h）； 2．术后各组实验犬血浆中SS浓度的变化：0h时8个实验组中各实验犬血浆中SS浓度差异不明显；在0.5h时各组实验犬血浆中SS浓度明显下降，考虑与其自身调节有关；随应激后时间延长SS浓度呈现递增趋势，且明显超过起始浓度（0h）； 3．术后A、B、F组末端回肠组织GnRH受体、SS受体的表达变化：采用Westernblot分析末端回肠组织GnRH受体、SS受体的表达发现，在急性应激状态下，GnRHR、SSTR表达均有增多的趋势；且GnRH受体、SS受体与对应GnRH浓度、SS浓度呈现正相关性。结论：1．颅脑火器伤下急性末端回肠炎血浆中GnRH浓度升高及其受体的表达增多； 2．颅脑火器伤下急性末端回肠炎血浆中SS浓度升高及其受体的表达增多； 3. GnRH、SS及其受体参与了应激对急性末端回肠炎的调控，且在一定时间内呈现强度正相关。
[Abstract]:Objective: to study the changes of gonadotropin releasing hormone (GnRH),) somatostatin (SS) in dogs and the expression of somatostatin (SS) receptor in experimental acute terminal colitis. To explore the relationship between stress and hormones and their receptors, and reveal the relationship between stress and intestinal diseases. Methods: forty healthy adult male mongrel dogs were randomly divided into 8 groups: normal blood glucose group (A group), hyperglycemia group (G group) and hyperglycemia group (B group). Normal blood glucose group (H group), normal blood glucose group (C group), craniocerebral firearm injury group (D group), craniocerebral firearm injury group (E group). The craniocerebral firearm injury group (group F) with high temperature and high humidity and hyperglycemia (group F) were given the model of craniocerebral firearm injury with pistols. The canine supine position was fixed on the operating table immediately after modeling, followed by tracheal intubation, ECG monitoring, cardiopulmonary resuscitation and other rescue measures to monitor the vital signs such as heart rate and blood pressure after injury. No other treatment except cardiac extrusion and artificial respiration sac extrusion. Abdominal surgery was performed immediately under aseptic conditions, ileocecal part was searched by laparotomy, and ileal tissue at the end of 1-3cm was immediately centrifuged and preserved, then the stump was anastomosed. According to the time gradient: 0 hours, 0.5 hours, 1 hour, 1.5 hours, 2 hours, 5 samples of peripheral blood were collected from each group of dogs, and then centrifuged immediately. In the process of delaying the life of experimental dogs as far as possible, intravenous fluid supply. Enzyme linked immunosorbent assay (Enzyme-LinkedImmunosorbent Assay,ELISA) was used to quantitatively detect the concentration of GnRH and SS in peripheral blood, and Westernblot was used to detect the expression of GnRH receptor and SS receptor at different time points in the terminal ileum tissue. Results: 1. The change of plasma GnRH concentration in each group: at 0 h, there was no significant difference in plasma GnRH concentration in 8 experimental groups. At 0.5 h, the concentration of GnRH in plasma of experimental dogs decreased obviously, which was related to their self-regulation, and the concentration of GnRH increased gradually with the prolongation of time after stress, and obviously exceeded the initial concentration (0 h). 2. The changes of plasma SS concentration in each group of experimental dogs: there was no significant difference in plasma SS concentration in each of the eight experimental groups at 0 h, and the SS concentration in plasma of each group decreased significantly at 0.5 h, which was related to its self-regulation. The concentration of SS increased with the prolongation of stress time and exceeded the initial concentration (0 h). 3. The expression of GnRH receptor and SS receptor in the terminal ileum of group A and B: the expression of GnRH receptor and SS receptor in terminal ileum were analyzed by Westernblot, and the expression of GnRHR,SSTR increased under acute stress. There was a positive correlation between GnRH receptor, SS receptor and corresponding GnRH concentration and SS concentration. Conclusion: 1. The concentration of GnRH and the expression of its receptor increased in the plasma of acute terminal colitis after craniocerebral firearm injury, 2. The concentration of SS and the expression of receptor in plasma of acute terminal gynecitis with craniocerebral firearm injury increased; 3. GnRH,SS and its receptors are involved in the regulation of stress on acute terminal colitis, and there is a positive correlation of intensity in a certain period of time.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R574

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