大鼠非酒精性脂肪性肝病MTP活性表达变化

发布时间：2018-11-11 20:46

【摘要】：目的探讨微粒体三酰甘油转移蛋白(MTP)对大鼠高脂性非酒精性脂肪性肝病(NAFLD)形成的影响。方法雄性Wistar大鼠随机分为对照组(NC)和高脂组(HF),NC组正常饲料喂养,HF组高脂饮食喂养12周,测定动物体重、肝湿重和肝指数,血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、血清和肝三酰甘油(TG)、总胆固醇(TCH)和病理组织学观察肝脂肪变情况;使用稳态寿命荧光光谱法检测肝和小肠微粒体MTP活性,Western blot检测肝和小肠微粒体MTP蛋白表达。结果与NC组比较,肝细胞呈明显脂肪变性,肝小叶内可见炎性细胞浸润和空泡变性;HF组大鼠肝湿重、肝指数,血清ALT、AST、TCH,肝TG、TCH值明显升高(P0.01,P0.05);肝和小肠微粒体MTP活性和蛋白表达量明显降低(P0.05)。结论高脂饮食诱导的大鼠NAFLD形成可能与抑制肝和小肠MTP活性及蛋白表达有关。
[Abstract]:Objective to investigate the effect of microsomal triglyceride transfer protein (MTP) on the formation of (NAFLD) in rats with hyperlipidemic nonalcoholic fatty liver disease. Methods male Wistar rats were randomly divided into control group (NC) and hyperlipidemia group (HF), NC group). The rats in HF group were fed with high fat diet for 12 weeks. The body weight, wet weight of liver, liver index and serum alanine aminotransferase (ALT), were measured. Aspartate transaminase (AST), serum and triglyceride (TG), total cholesterol (TCH) and histopathology were observed. MTP activity of liver and small intestine microsomes was detected by steady-state lifetime fluorescence spectrometry. The expression of MTP protein in liver and small intestine microsomes was detected by, Western blot. Results compared with NC group, hepatic cells showed steatosis, inflammatory cell infiltration and vacuolar degeneration were found in hepatic lobules, liver wet weight, liver index and serum TG,TCH value of ALT,AST,TCH, were significantly increased in HF group (P 0.05). Liver and small intestine microsomal MTP activity and protein expression decreased significantly (P0.05). Conclusion the formation of NAFLD in rats induced by high fat diet may be related to the inhibition of MTP activity and protein expression in liver and small intestine.
【作者单位】：安徽医学高等专科学校临床医学系;安徽医科大学第一附属医院消化内科;
【基金】：安徽高校省级自然科学研究项目(KJ2008B309) 安徽省卫生厅医学科研基金项目(072A008)
【分类号】：R575

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