水通道蛋白2在梗阻性黄疸大鼠肾脏表达变化的实验研究

发布时间：2018-11-14 21:23

【摘要】：目的:明确水通道蛋白2(AQP2)在梗阻性黄疸大鼠肾脏表达变化及其与尿素氮(BUN)和肌酐(Cr)变化的关系。方法:采用手术结扎胆总管的方法造成实验大鼠梗阻性黄疸模型,于术后3、5、7、10、14 d处死大鼠取血和肾脏标本,检测血浆直接胆红素、尿素氮和肌酐值,光镜下观察肾脏病理形态学,免疫组化方法检测肾脏APQ2表达。结果:实验组3 d时光镜下见肾集合管上皮细胞排列不规则肿胀,5 d时可见细胞水样空泡变性、并且梗阻性黄疸组AQP2表达较对照组明显减少。7 d时见部分细胞脱落、间质有炎性细胞浸润,10和14 d时见局灶上皮细胞坏死、胞浆崩解、间质有大量炎性细胞浸润。水通道蛋白2表达在梗阻5 d后较对照组明显减少(P0.05),随着梗阻时间延长,其表达减少愈加明显。AQP2表达减少的同时,肾形态学损害在光镜下从肾集合管形态学改变得到证实。梗阻性黄疸组术后10 d Cr开始升高,梗阻14 d后BUN开始升高。结论:AQP2的减少表明肾功能发生损害,AQP2减少在前,CR和BUN增加在后,所以AQP2作为肾功能损害的早期检测指标是很有意义的。
[Abstract]:Aim: to investigate the expression of aquaporin 2 (AQP2) in the kidney of obstructive jaundice rats and its relationship with urea nitrogen (BUN) and creatinine (Cr). Methods: the experimental rat model of obstructive jaundice was established by ligating the common bile duct. The blood and kidney samples were collected from the rats on the 14th day after operation, and the levels of plasma direct bilirubin, urea nitrogen and creatinine were measured. The pathological morphology of kidney was observed under light microscope and the expression of APQ2 in kidney was detected by immunohistochemical method. Results: in the experimental group, the epithelial cells of the renal collecting duct were arranged irregularly under microscope for 3 days, and the vacuolar degeneration was observed at 5 days. The expression of AQP2 in the obstructive jaundice group was significantly lower than that in the control group. At 7 days, some cells were abscission. The interstitial cells were infiltrated with inflammatory cells. At 10 and 14 days, focal epithelial cells were necrotic, cytoplasm disintegrated, and a large number of inflammatory cells were infiltrated in the interstitial. The expression of aquaporin-2 was significantly decreased 5 days after obstruction than that of control group (P0.05), and the expression of aquaporin-2 decreased significantly with the prolongation of obstruction time, while the expression of AQP2 decreased at the same time. Renal morphological damage was confirmed by morphological changes of renal collecting duct under light microscope. In the obstructive jaundice group, Cr began to increase 10 days after operation and BUN began to increase after 14 days of obstruction. Conclusion: the decrease of AQP2 indicates the impairment of renal function, the decrease of AQP2, the increase of CR and BUN, so AQP2 is an early indicator of renal dysfunction.
【作者单位】：沈阳医学院附属第二医院普外科;中国医科大学附属盛京医院胆道疝减肥外科;
【分类号】：R575.5

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