PPARγ激动剂干预对急性胰腺炎小鼠肝损伤的影响

发布时间：2018-11-17 21:12

【摘要】：目的探讨过氧化物酶体增殖物激活受体γ(PPARγ)激动剂罗格列酮对急性胰腺炎小鼠肝损伤的影响并对其机制进行初步研究。方法 72只健康雄性昆明小鼠分为3组,急性胰腺炎组(AP组)、罗格列酮预处理组(AP-ROS组)和生理盐水组(NS组),每组24只。分别于建模后6h、12h和24h处死小鼠,全自动生化分析仪检测血清淀粉酶、丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)水平。运用RT-PCR方法检测肝脏组织NF-κB和PPARγmRNA表达,应用Western blot技术检测肝脏组织PPARγ和NF-κB p65蛋白表达。结果小鼠血清淀粉酶、ALT和AST水平在相对应的各时间点AP组比NS组明显升高(P0.01),AP-ROS组较AP组明显降低(P0.01)。AP组肝脏组织PPARγmRNA和蛋白表达在造模后6h和12h均低于NS组(P0.05);AP-ROS组PPARγmRNA和蛋白表达在各时间点均明显高于AP组和NS组(P0.01)。AP组肝脏组织NF-κB mRNA和NF-κB p65蛋白表达水平在各时间点与AP-ROS组和NS组相比较均升高(P0.01)。结论 NF-κB与小鼠急性胰腺炎肝损伤有明显关系,PPARγ在肝损伤中的表达受到抑制;罗格列酮在AP早期能增强PPARγ表达,抑制NF-κB的表达。
[Abstract]:Objective to investigate the effect of rosiglitazone (rosiglitazone), a peroxisome proliferator activated receptor 纬 (PPAR 纬) agonist, on liver injury in mice with acute pancreatitis. Methods 72 healthy male Kunming mice were divided into 3 groups: acute pancreatitis group (AP group), rosiglitazone preconditioning group (AP-ROS group) and normal saline group (NS group), with 24 rats in each group. The serum amylase, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by automatic biochemical analyzer. The expression of NF- 魏 B and PPAR 纬 mRNA in liver tissue was detected by RT-PCR method, and the expression of PPAR 纬 and NF- 魏 B p65 protein in liver tissue was detected by Western blot technique. Results the levels of serum amylase, ALT and AST were significantly higher in AP group than in NS group at each time point (P0.01). The expression of PPAR 纬 mRNA and protein in liver tissue of AP-ROS group was significantly lower than that of AP group (P0.01). AP group was significantly lower than that of NS group at 6 h and 12 h after modeling (P0.05). The expression of PPAR 纬 mRNA and protein in AP-ROS group was significantly higher than that in AP group and NS group at all time points (P0.01). The expression levels of NF- 魏 B mRNA and NF- 魏 B p65 protein in liver tissue in P0.01). AP group were significantly higher than those in AP-ROS group and NS group at each time point (P0.01). Conclusion NF- 魏 B has a significant relationship with liver injury in mice with acute pancreatitis, and the expression of PPAR 纬 in liver injury is inhibited, and rosiglitazone can enhance the expression of PPAR 纬 and inhibit the expression of NF- 魏 B in the early stage of AP.
【作者单位】：济南军区第456医院;山东大学医学院;
【基金】：山东省科技发展计划(2006GG2202021) 山东省自然科学基金(Y2005C29)
【分类号】：R576

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