门静脉灌注80%乙醇建立猪肝硬化门静脉高压模型

发布时间：2018-11-27 06:38

【摘要】：目的探讨门静脉灌注80%乙醇建立猪肝硬化门静脉高压动物模型的可行性。方法选取健康贵州小型香猪13只,随机分为3组:实验1组(5只)、2组(5只)及对照组(3只),实验1、2组门静脉分别灌注80%乙醇5ml及10ml,对照组灌注生理盐水10ml,对所有实验猪于灌注前、灌注后即刻、灌注后6周行直接门静脉造影测量门静脉压力及直径;灌注前及灌注后6h内、术后1~6周行肝组织活检;灌注前及灌注后6周分别行增强CT;灌注后6周解剖所有实验猪,对肝脏各叶标本进行组织学检查。结果实验1组及对照组实验猪灌注前、后门静脉直径及压力的差异无统计学意义(P均0.05)。实验2组灌注后即刻、灌注后6周与灌注前比较,门静脉直径及压力的差异均有统计学意义(P均0.05)。实验1、2组灌注后所有实验猪均形成肝纤维化,灌注后6周实验1组2只、实验2组5只纤维化METAVIR评分达4级。结论经门静脉灌注80%乙醇更适宜构建肝硬化门静脉高压动物模型。
[Abstract]:Objective to investigate the feasibility of establishing porcine cirrhotic portal hypertension model by portal vein perfusion with 80% ethanol. Methods Thirteen healthy Guizhou miniature pigs were randomly divided into three groups: experimental group (n = 5), experimental group (n = 5) and control group (n = 3). Portal vein pressure and diameter were measured by direct portography before perfusion, immediately after perfusion and 6 weeks after perfusion. Liver biopsy was performed before and 6 hours after perfusion, and liver biopsy was performed 6 weeks before and 6 weeks after perfusion with enhanced CT; perfusion. Results there was no significant difference in the diameter and pressure of portal vein between experimental group 1 and control group before and after perfusion (P 0.05). There were significant differences in the diameter and pressure of portal vein between the two groups immediately after perfusion and 6 weeks after perfusion (P 0.05). In experiment 1 and group 2, hepatic fibrosis was formed in all the pigs after perfusion. The METAVIR score of fibrosis in experimental group 1 and group 2 reached grade 4 6 weeks after perfusion. Conclusion Perfusion of 80% ethanol through portal vein is more suitable for the establishment of portal hypertension model of liver cirrhosis.
【作者单位】：首都医科大学北京世纪坛医院介入治疗科;
【分类号】：R575.2;R-332

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