肿瘤坏死因子-α在小鼠酒精性肝损伤过程中的表达及意义

发布时间：2018-11-29 10:31

【摘要】：目的研究肿瘤坏死因子-α(TNF-α)在小鼠酒精性肝损伤过程中的表达与意义。方法将50只健康雄性小鼠按照体重随机分为2组:对照组(n=10),模型组(n=40)。用56°红星二锅头经酒精灌胃(15 m L·kg-1)诱导小鼠肝损伤1,2,3,4周,建立小鼠酒精性肝损伤模型,分别于0(对照组小鼠)与1,2,3,4周,各取10只小鼠于眼球取血,用赖式法检测血清谷草转氨酶(AST)的活性;以颈椎脱臼法处死小鼠,取小鼠肝,用蛋白印迹法测定肝组织TNF-α的表达量。结果小鼠肝AST酶的活性在0,1,2,3,4周分别为(112±22),(126±24),(967±30),(1010±35),(206±23)U·L~(-1),与对照组相比,差异均有统计学意义(均P0.05)。模型组的小鼠肝AST酶的活性在第1周缓慢上升,第2周迅速上升,于第3周达到高峰,第4周开始下降,但仍高于对照组。TNF-α相对积分光密度在0,1,2,3,4周分别为1.15±0.12,1.10±0.08,2.13±0.16,2.16±0.15,1.16±0.11,与对照组相比,第1周略有下降,第2周迅速上升,第3周达到高峰,第4周出现下降,仍高于对照组,差异均有统计学意义(均P0.01)。结论在酒精灌胃诱导的小鼠肝损伤的进程早期,TNF-α促使肝细胞的损伤和坏死,并参与了后期肝的修复再生。
[Abstract]:Objective to study the expression and significance of tumor necrosis factor- 伪 (TNF- 伪) in alcoholic liver injury in mice. Methods 50 healthy male mice were randomly divided into two groups according to their body weight: control group (n = 10) and model group (n = 40). Mice liver injury induced by 56 掳Hongxing Erguotou was induced by alcohol (15 mL kg-1) for 3 weeks. The model of alcoholic liver injury was established in mice. 10 mice were taken from eyeball for 4 weeks. The activity of serum glutamic oxaloacetic transaminase (AST) was detected by the method of Lai. The mice were killed by cervical dislocations, and the expression of TNF- 伪 in the liver was determined by Western blot. Results the activity of AST enzyme in liver of mice was (112 卤22), (, 126 卤24), (, 967 卤30), (, 1010 卤35), (206 卤23) U L ~ (-1) at 4 weeks, which was significantly different from that of control group (P0.05). In the model group, the activity of liver AST enzyme increased slowly at the first week, rose rapidly at the second week, reached the peak at the third week, and began to decrease at the fourth week, but it was still higher than that in the control group. In the 4th week, the values were 1.15 卤0.12, 1.10 卤0.08, 2.13 卤0.16, 2.16 卤0.15, 1.16 卤0.11, respectively. Compared with the control group, it decreased slightly in the first week, increased rapidly in the second week, reached the peak at the third week, and decreased at the fourth week, which was still higher than that in the control group. The difference was statistically significant (all P 0.01). Conclusion in the early stage of liver injury induced by alcohol, TNF- 伪 promotes the damage and necrosis of liver cells and participates in the repair and regeneration of the later stage of liver.
【作者单位】：河南科技大学医学院肝脏损伤与修复分子医学重点实验室;
【基金】：国家自然科学基金资助项目(81201558) 河南省科技攻关基金资助项目(172102310693) 河南科技大学创新团队基金资助项目(2015XTD003)
【分类号】：R575

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