微量元素锶对非酒精性脂肪肝大鼠的防治作用研究

发布时间：2019-01-04 17:29

【摘要】：目的探讨微量元素锶对大鼠脂代谢紊乱的改善及非酒精性脂肪肝的防治作用及可能机制。方法将50只SD大鼠分为5组,对照组用普通饲料,其余4组用高脂饲料,共喂养13周。18 mg/L锶组和36 mg/L锶组在后9周分别给予18mg/L与36mg/L的锶水,辛伐他汀组于后4周每天给予10mg/kg辛伐他汀灌胃。第14周末处死大鼠,检测肝指数、血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)及肝组织TG和TC含量,肝组织冰冻切片,油红O染色观察脂肪样变及其分布情况。结果与对照组比较,脂肪肝模型组的肝指数、肝TC、肝TG、血清TC、LDL-C均升高(P0.05);与脂肪肝模型组比较,18 mg/L锶组大鼠血清TC、LDL-C、HDL-C也降低(P0.05);36mg/L锶组肝指数、肝TC、肝TG、血清TC、LDL-C均降低(P0.05)。油红O染色结果显示:脂肪肝模型组肝组织含有大量的红染脂肪颗粒;18mg/L锶组、36mg/L锶组以及辛伐他汀组红染颗粒含量均较脂肪肝模型组有不同程度减少。结论长期较高浓度的微量元素锶摄入具有改善大鼠脂代谢紊乱,防治非酒精性脂肪肝的作用。
[Abstract]:Objective to investigate the effect of strontium on the improvement of lipid metabolism disorder and the prevention and treatment of non-alcoholic fatty liver in rats. Methods Fifty SD rats were divided into 5 groups. The control group was fed with normal diet, the other 4 groups were fed with high fat diet for 13 weeks. The 18 mg/L Sr group and 36 mg/L Sr group were given 18mg/L and 36mg/L strontium water respectively at 9 weeks after feeding. Simvastatin group was given 10mg/kg simvastatin daily 4 weeks later. Rats were killed at the end of the 14th week. Liver index and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), were measured. Low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), contents of TG and TC in liver tissue, frozen sections of liver tissue, oil red O staining were used to observe the fatty changes and their distribution. Results compared with the control group, the liver index and serum TC,LDL-C of TC, liver TG, in fatty liver model group were increased (P0.05). Compared with fatty liver model group, serum TC,LDL-C,HDL-C of 18 mg/L Sr group was also decreased (P0.05), liver index of 36mg/L strontium group and serum TC,LDL-C of liver TC, liver TG, were all decreased (P0.05). The results of oil red O staining showed that there were a lot of red stained fat particles in liver tissue of fatty liver model group, and the content of red stained granules in 18mg/L strontium group, 36mg/L strontium group and simvastatin group were decreased to some extent compared with fatty liver model group. Conclusion Strontium intake with high concentration for a long time can improve lipid metabolism disorder and prevent and cure non-alcoholic fatty liver in rats.
【作者单位】：西南医科大学基础医学院;
【分类号】：R575.5

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