GCKR rs3817588、rs780094位点单核苷酸多态性与非酒精性脂肪肝的相关性研究

发布时间：2019-01-04 19:50

【摘要】：目的探讨葡萄糖激酶调节蛋白(GCKR)基因rs3817588和rs780094位点单核苷酸多态性(SN P)与非酒精性脂肪肝(NAFLD)发病易感性的关系。方法根据B超结果,筛选纳入NAFLD组132例和对照组252例,采集其病史,采集血液样本,分析血糖、血脂等血生化指标,全血中提取DNA,采用聚合酶链反应(PCR)、MassARRAY时间飞行质谱技术分析GCKR rs3817588、rs780094位点基因型。结果NAFLD组与正常对照组GCKR rs3817588基因型频率分布无显著差异(P0.05),但其等位基因频率分布存在统计学意义(P0.05),等位基因C使发生NAFLD的风险度下降29.7%(OR=0.703,95%CI:0.500~0.981),而且,等位基因C携带者尿酸水平明显降低。GCKR rs780094位点的基因型频率和等位基因频率分布在NAFLD组与正常对照组间无差异(P0.05),各基因型间生化指标和一般指标的变化无统计差异,经校正年龄、性别后,NAFLD的风险度无明显变化。结论GCKR rs3817588 C等位基因与低血尿酸有关,并且降低NAFLD的发病风险,但GCKRrs780094位点基因多态性与NAFLD的发生无明显相关性。
[Abstract]:Objective to investigate the relationship between (GCKR) gene rs3817588 and rs780094 single nucleotide polymorphism (SN P) (SNP) and (NAFLD) susceptibility in non-alcoholic fatty liver. Methods according to the results of B-ultrasound, 132 cases of NAFLD group and 252 cases of control group were selected. Their history, blood samples, blood glucose, blood lipid and other biochemical indexes were collected. DNA, was extracted from the whole blood by polymerase chain reaction (PCR),). GCKR rs3817588,rs780094 locus genotypes were analyzed by MassARRAY time flight mass spectrometry. Results there was no significant difference in the frequency distribution of GCKR rs3817588 genotype between the NAFLD group and the normal control group (P0.05), but the allele frequency distribution was statistically significant (P0.05). Allele C reduced the risk of NAFLD by 29. 7% (OR=0.703,95%CI:0.500~0.981), and, The level of uric acid in allelic C carriers was significantly decreased. There was no significant difference in genotype frequency and allele frequency of GCKR rs780094 between NAFLD group and normal control group (P0.05). There was no statistical difference in the changes of biochemical indexes and general indexes among genotypes. After adjusting for age and sex, there was no significant change in the risk degree of NAFLD. Conclusion the allele of GCKR rs3817588 C is associated with hypouric acid and decreases the risk of NAFLD, but the polymorphism of GCKRrs780094 locus is not associated with the occurrence of NAFLD.
【作者单位】：苏州大学公共卫生学院营养与食品卫生教研室;苏州市相城区第三人民医院;
【基金】：国家自然科学基金面上项目(No.81372980)
【分类号】：R575.5

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