胃粘膜非典型增生中微环境蛋白质的相互作用
发布时间：2019-01-05 14:01
【摘要】：微环境在胃癌发病过程中发挥重要作用。了解胃粘膜早期癌变的分子机制,对防治胃癌具有十分重要的意义。为了解胃粘膜非典型增生过程中,微环境中蛋白质的相互作用及调节机制,采用激光捕获显微切割(laser capture microdissection,LCM)技术,纯化正常胃粘膜组织(normal gastric mucosa tissue,NGM)和胃粘膜非典型增生(gastric mucosal atypical hyperplasia,GMAH)间质,通过同位素标记定量蛋白质组学技术分析,鉴定NGM和GMAH间质的差异表达蛋白质。利用生物信息学软件,分析NGM和GMAH间质差异表达蛋白质的相互作用及其联系。共鉴定出165个GMAH间质差异表达蛋白质,其中GMAH组织中表达上调者99个,下调者66个。它们涉及一些与肿瘤相关的信号通路,如p53信号通路、MAPK信号通路、细胞周期与凋亡等信号通路,且与细胞生长、增殖、凋亡和体液免疫应答等生物学过程有关。这些差异表达蛋白质,在STRING网络中呈现相互作用,两两间相互联系。本文的研究提示,胃粘膜非典型增生微环境中存在S100A6和SOD3等蛋白质间的相互作用,它们通过影响p53信号通路、MAPK信号通路、细胞周期与凋亡等信号通路,在胃癌发病过程中发挥作用。
[Abstract]:Microenvironment plays an important role in the pathogenesis of gastric cancer. It is very important to understand the molecular mechanism of early canceration of gastric mucosa in prevention and treatment of gastric cancer. In order to understand the interaction and regulation mechanism of protein in microenvironment during atypical hyperplasia of gastric mucosa, the (normal gastric mucosa tissue, of normal gastric mucosa was purified by laser capture microdissection (laser capture microdissection,LCM) technique. NGM) and (gastric mucosal atypical hyperplasia,GMAH) were used to identify the differentially expressed proteins between NGM and GMAH. The interaction and relationship of differentially expressed proteins between NGM and GMAH were analyzed by bioinformatics software. A total of 165 differentially expressed GMAH proteins were identified, of which 99 were up-regulated in GMAH and 66 were down-regulated. They involve some signal pathways related to tumor, such as p53 signaling pathway, MAPK signaling pathway, cell cycle and apoptosis signaling pathway, and also related to cell growth, proliferation, apoptosis and humoral immune response. These differentially expressed proteins, which interact in STRING networks, are related to each other. The present study suggests that there are interactions between S100A6 and SOD3 in gastric mucosal dysplasia microenvironment, which affect p53 signaling pathway, MAPK signal pathway, cell cycle and apoptosis signaling pathway. Play a role in the pathogenesis of gastric cancer.
【作者单位】： 南华大学医学院肿瘤研究所肿瘤细胞与分子病理学湖南省重点实验室;南华大学附属第一医院;邵阳医学高等专科学校附属医院儿科;南华大学医学院;
【基金】：湖南省高校创新平台基金(No.12K094,No.13K083) 湖南省科技厅项目(No.2015SK20203) 湖南省教育厅课题(No.11C1112) 湖南省卫生厅课题(No.B2013-048,No.2014-163) 湖南省研究生科研创新项目(No.CX2016B478)~~
【分类号】：R573
，
本文编号：2401860
[Abstract]:Microenvironment plays an important role in the pathogenesis of gastric cancer. It is very important to understand the molecular mechanism of early canceration of gastric mucosa in prevention and treatment of gastric cancer. In order to understand the interaction and regulation mechanism of protein in microenvironment during atypical hyperplasia of gastric mucosa, the (normal gastric mucosa tissue, of normal gastric mucosa was purified by laser capture microdissection (laser capture microdissection,LCM) technique. NGM) and (gastric mucosal atypical hyperplasia,GMAH) were used to identify the differentially expressed proteins between NGM and GMAH. The interaction and relationship of differentially expressed proteins between NGM and GMAH were analyzed by bioinformatics software. A total of 165 differentially expressed GMAH proteins were identified, of which 99 were up-regulated in GMAH and 66 were down-regulated. They involve some signal pathways related to tumor, such as p53 signaling pathway, MAPK signaling pathway, cell cycle and apoptosis signaling pathway, and also related to cell growth, proliferation, apoptosis and humoral immune response. These differentially expressed proteins, which interact in STRING networks, are related to each other. The present study suggests that there are interactions between S100A6 and SOD3 in gastric mucosal dysplasia microenvironment, which affect p53 signaling pathway, MAPK signal pathway, cell cycle and apoptosis signaling pathway. Play a role in the pathogenesis of gastric cancer.
【作者单位】： 南华大学医学院肿瘤研究所肿瘤细胞与分子病理学湖南省重点实验室;南华大学附属第一医院;邵阳医学高等专科学校附属医院儿科;南华大学医学院;
【基金】：湖南省高校创新平台基金(No.12K094,No.13K083) 湖南省科技厅项目(No.2015SK20203) 湖南省教育厅课题(No.11C1112) 湖南省卫生厅课题(No.B2013-048,No.2014-163) 湖南省研究生科研创新项目(No.CX2016B478)~~
【分类号】：R573
，
本文编号：2401860
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