乙醇性脂肪肝模型大鼠早期生长反应因子1表达及水飞蓟素的干预

发布时间：2019-01-05 16:50

【摘要】：背景:促炎性转录因子以及炎性因子在乙醇性肝病的发生中起着重要作用,而早期生长反应因子1正是炎症启动的关键因子之一。目的:观察并分析水飞蓟素对乙醇性脂肪肝模型大鼠早期生长反应因子1的影响。方法:将大鼠采用高脂饮食联合乙醇灌胃方式建立脂肪肝动物模型,每次灌胃后分别给予水飞蓟素高、低剂量(200,100 mg/kg)干预,并设正常组进行对照,共灌胃8周。结果与结论:血清学指标检测和苏木精-伊红染色结果显示,与模型组相比,水飞蓟素高剂量组大鼠体质量增高(P0.05),血清谷草转氨酶、谷丙转氨酶活性、肝组织早期生长反应因子1和肿瘤坏死因子α的表达降低(P0.05),病理分级结果均占优(P0.01)。结果证实,高剂量水飞蓟素能有效保护大鼠肝功能,减少肝功能损害的发生,可能与水飞蓟素抑制大鼠体内早期生长反应因子1,从而减少肿瘤坏死因子α的形成有关,但具体机制尚待进一步研究。
[Abstract]:Background: proinflammatory transcription factors and inflammatory factors play an important role in the pathogenesis of alcoholic liver disease, and early growth response factor 1 is one of the key factors in the initiation of inflammation. Aim: to observe and analyze the effect of silymarin on early growth response factor 1 in rats with alcoholic fatty liver. Methods: the fatty liver model was established in rats with high fat diet and ethanol. The rats were treated with silymarin at high and low doses (200100 mg/kg) for 8 weeks. The rats in the control group were treated with high and low doses of silymarin for 8 weeks. Results and conclusion: compared with the model group, the results of serological indexes and hematoxylin-eosin staining showed that the body weight, serum glutamic oxaloacetic transaminase and alanine aminotransferase activity in silymarin high dose group were higher than those in model group. The expression of early growth response factor 1 and tumor necrosis factor 伪 in liver tissue was decreased (P0.05), and the pathological grade was superior (P0.01). The results showed that high dose silymarin could effectively protect the liver function of rats and reduce the occurrence of liver function damage, which might be related to the inhibition of silymarin on the early growth response factor 1 and the reduction of the formation of tumor necrosis factor 伪 in rats. However, the specific mechanism remains to be further studied.
【作者单位】：黄陂区人民医院感染性疾病科;
【分类号】：R575.5;R-332

【参考文献】