云南某高校南亚留学生、国内学生心理因素与消化道优势菌群相关性研究
发布时间：2019-01-11 08:34
【摘要】：目的本研究以在校医学本科生为研究对象,应用实时荧光定量PCR法检测其消化道菌群(口腔菌群和肠道菌群)中优势菌群的数量,重点了解南亚留学生与国内学生消化道优势菌群数量的差异,并研究口腔菌群与肠道菌群数量间的差异。探讨影响消化道优势菌群数量的因素,分析消化道优势菌群数量与心理因素的相关关系,为提高南亚留学生在华学习生活质量提供参考,并为全面深入了解消化道优势菌群与其影响因素间的关系将如何维持消化道微生态系统平衡提供科学依据。方法1、筛选研究对象及调查取样:按照严格的纳入及排除标准,选取2014级医学专业的50名南亚留学生及50名国内学生为研究对象。采用抑郁-焦虑-压力量表、艾森克人格问卷简式量表、匹兹堡睡眠质量指数量表对研究对象的心理健康状况进行评价。并采集所有入选者的粪便样本和口腔拭子样本,提取基因组DNA,均一化核酸浓度至20μg/m L。2、SYBR GreenⅠ实时荧光定量PC R质粒标准品的制备:利用具有各菌群种属特异性基因的16Sr DNA作为扩增区设计引物进行PCR扩增,将扩增的目标片段与质粒载体连接转化到大肠杆菌中保存,经过测序鉴定后,将质粒定量并梯度稀释作为绝对定量的标准品,建立标准曲线。3、消化道菌群定量检测:应用实时荧光定量PCR法检测两组学生的粪便样本及口腔拭子样本中总菌群、拟杆菌属、双歧杆菌属、乳酸杆菌属、大肠埃希菌、肠球菌属数量。4、资料整理与分析:采用SPSS17.0统计学软件分析南亚留学生和国内学生消化道优势菌群数量间的差异,以及消化道优势菌群数量与心理因素之间的相关关系。结果1、两组人群的问卷调查结果分析:两组人群在年龄、性别、体质指数(BMI)、分娩方式、运动习惯、排便习惯等方面差异均无统计学意义(P0.05),但南亚留学生组在抑郁、焦虑、压力3个分量表的得分明显高于国内学生组(P0.05),差异均具有统计学意义。2、两组人群肠道菌群定量分析:与国内学生组比,南亚留学生组粪便样本中总菌群、拟杆菌属、双歧杆菌属、乳酸杆菌属比国内学生组的数量少(P0.05),大肠埃希菌、肠球菌属比国内学生组的数量多(P0.05),差异均具有统计学意义。3、两组人群口腔菌群定量分析:与国内学生组相比,南亚留学生组口腔拭子样本中拟杆菌属、双歧杆菌属、乳酸杆菌属比国内学生组的数量少(P0.05),大肠埃希菌、肠球菌属比国内学生组的数量多(P0.05),差异均具有统计学意义。4、口腔菌群与肠道菌群定量比较分析:口腔菌群(口腔拭子样本)比肠道菌群(粪便样本)的总菌群、拟杆菌属、双歧杆菌属、乳酸杆菌属、大肠埃希菌的数量均少(P0.05),而肠球菌属为口腔菌群比肠道菌群数量多(P0.05),差异均具有统计学意义。5、两组人群肠道定植抗力分析:两组人群肠道定植抗力B/E值除个别外均大于1,南亚留学生组与国内学生组相比B/E值较低(P0.05),差异具有统计学意义。6、消化道菌群与心理因素的相关性分析:粪便样本中大肠埃希菌数量与抑郁量表、压力量表的得分均成正相关(r=0.243,P0.05;r=0.217,P0.05);口腔拭子样本中大肠埃希菌数量与抑郁量表的得分成正相关(r=0.250,P0.05),差异均具有统计学意义。结论1、该校南亚留学生与国内学生均具有良好的肠道微生态,但国内学生的肠道定植抗力强于南亚留学生。2、该校南亚留学生与国内学生在消化道优势菌群数量上存在一定的差异,表现为南亚留学生消化道的拟杆菌属、双歧杆菌属、乳酸杆菌属数量比国内学生少,而大肠埃希菌、肠球菌属数量比国内学生多。3、该校南亚留学生整体心理状况良好,但仍有个别学生的抑郁、焦虑、压力症状严重,需要相关管理人员和心理学者予以密切关注,预防严重心理问题的出现。4、消化道菌群中大肠埃希菌的数量可能与心理因素之间存在一定的相关性,但具体作用机制有待进一步的研究。
[Abstract]:Objective To study the number of dominant bacteria in the digestive tract bacteria group (oral cavity group and intestinal flora) by real-time fluorescence quantitative PCR method, and to study the difference of the number of dominant bacteria in the digestive tract of the students in South Asia and the domestic students. The differences between the number of intestinal flora and the number of intestinal flora were also studied. The factors influencing the number of the dominant bacteria in the digestive tract were discussed, the relationship between the number of dominant bacteria in the digestive tract and the psychological factors was analyzed, and the reference for the improvement of the quality of life of the students in South Asia was provided. In order to provide a scientific basis for how to maintain the balance of microecosystem in the digestive tract in order to get a thorough understanding of the relationship between the flora of the digestive tract and its influencing factors. Method 1. Filter the study object and investigate the sampling: according to the strict inclusion and exclusion criteria, select 50 South Asian students and 50 national students in the medical profession in 2014 as the study object. The mental health status of the study object was evaluated by the depression-anxiety-pressure scale, the Eysenck Personality Questionnaire (EPQ) and the Pittsburgh Sleep Quality (Pittsburgh) sleep quality. and collecting the stool sample and the oral swab sample of all the selected persons, extracting the genomic DNA, homogenizing the nucleic acid concentration to 20 & mu; g/ mL. 2, and preparing the SYBR Green I real-time fluorescent quantitative PC R plasmid standard product. PCR amplification is carried out by using 16Sr DNA with specific genes of each bacterium group as an amplification region, and the amplified target fragment and the plasmid vector are connected and transformed into E. coli for preservation; after sequencing and identification, the plasmid is quantitatively and gradient diluted to be used as an absolute quantitative standard, Establishment of the standard curve. 3. Quantitative detection of the intestinal flora: The real-time fluorescence quantitative PCR was applied to detect the total bacterial group, the genus Bacteroides, the genus Bifidobacterium, the genus Lactobacillus, the Escherichia coli and the genus Enterococcus in both groups. The statistical software of SPSS17. 0 is used to analyze the difference between the number of the dominant bacteria in the digestive tract of the students in South Asia and the domestic students, as well as the relationship between the number of dominant bacteria in the digestive tract and the psychological factors. Results 1. The results of the questionnaire of the two groups showed no significant difference in age, sex, body mass index (BMI), delivery mode, exercise habit, and defecation habit (P0.05). The scores of three components in pressure were significantly higher than those in the domestic group (P0.05), and the difference was statistically significant. 2. The quantitative analysis of the intestinal flora in the two groups: the total bacteria group, the genus Bacteroides, the genus Bifidobacterium, The number of lactobacillus in the group was less than that in the domestic group (P0.05), and the number of E. coli and Enterococcus was much higher than that in the domestic group (P0.05). The number of Bacteroides, Bifidobacterium, and Lactobacillus in the oral swab samples of the foreign students in South Asia was less than that in the domestic group (P0.05), and the number of E. coli and Enterococcus was much higher than that in the domestic group (P0.05). The difference was statistically significant. The quantitative comparison between the population of the oral cavity and the intestinal flora: the number of the total bacteria group (the oral swab sample) of the oral cavity group (the oral swab sample) is lower than that of the intestinal flora (stool sample), and the number of the Bacteroides, the genus Bifidobacterium, the genus Lactobacillus and the Escherichia coli is less (P0.05). The intestinal colonization resistance of the two groups was higher than that of the two groups (P0.05). The correlation between the number of Escherichia coli and the depression scale and the pressure scale in the stool sample was positively correlated (r = 0.243, P0.05; r = 0.217, P0.05); There was a positive correlation between the number of Escherichia coli and the depression scale in the oral swab samples (r = 0.250, P0.05). Conclusion 1. The foreign students in South Asia and the domestic students have good intestinal microecology, but the intestinal colonization resistance of the domestic students is stronger than that of the students in South Asia. The number of Bacteroides, Bifidobacterium, and Lactobacillus in the digestive tract of the international students in South Asia is less than that of the domestic students, while the number of Escherichia coli and Enterococcus is more than that of the domestic students. 3. The students in South Asia are in good health, but still have the depression and anxiety of individual students. There is a certain correlation between the number of Escherichia coli in the digestive tract and the psychological factors, but the specific mechanism of action is to be further studied.
【学位授予单位】：大理大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R57
[Abstract]:Objective To study the number of dominant bacteria in the digestive tract bacteria group (oral cavity group and intestinal flora) by real-time fluorescence quantitative PCR method, and to study the difference of the number of dominant bacteria in the digestive tract of the students in South Asia and the domestic students. The differences between the number of intestinal flora and the number of intestinal flora were also studied. The factors influencing the number of the dominant bacteria in the digestive tract were discussed, the relationship between the number of dominant bacteria in the digestive tract and the psychological factors was analyzed, and the reference for the improvement of the quality of life of the students in South Asia was provided. In order to provide a scientific basis for how to maintain the balance of microecosystem in the digestive tract in order to get a thorough understanding of the relationship between the flora of the digestive tract and its influencing factors. Method 1. Filter the study object and investigate the sampling: according to the strict inclusion and exclusion criteria, select 50 South Asian students and 50 national students in the medical profession in 2014 as the study object. The mental health status of the study object was evaluated by the depression-anxiety-pressure scale, the Eysenck Personality Questionnaire (EPQ) and the Pittsburgh Sleep Quality (Pittsburgh) sleep quality. and collecting the stool sample and the oral swab sample of all the selected persons, extracting the genomic DNA, homogenizing the nucleic acid concentration to 20 & mu; g/ mL. 2, and preparing the SYBR Green I real-time fluorescent quantitative PC R plasmid standard product. PCR amplification is carried out by using 16Sr DNA with specific genes of each bacterium group as an amplification region, and the amplified target fragment and the plasmid vector are connected and transformed into E. coli for preservation; after sequencing and identification, the plasmid is quantitatively and gradient diluted to be used as an absolute quantitative standard, Establishment of the standard curve. 3. Quantitative detection of the intestinal flora: The real-time fluorescence quantitative PCR was applied to detect the total bacterial group, the genus Bacteroides, the genus Bifidobacterium, the genus Lactobacillus, the Escherichia coli and the genus Enterococcus in both groups. The statistical software of SPSS17. 0 is used to analyze the difference between the number of the dominant bacteria in the digestive tract of the students in South Asia and the domestic students, as well as the relationship between the number of dominant bacteria in the digestive tract and the psychological factors. Results 1. The results of the questionnaire of the two groups showed no significant difference in age, sex, body mass index (BMI), delivery mode, exercise habit, and defecation habit (P0.05). The scores of three components in pressure were significantly higher than those in the domestic group (P0.05), and the difference was statistically significant. 2. The quantitative analysis of the intestinal flora in the two groups: the total bacteria group, the genus Bacteroides, the genus Bifidobacterium, The number of lactobacillus in the group was less than that in the domestic group (P0.05), and the number of E. coli and Enterococcus was much higher than that in the domestic group (P0.05). The number of Bacteroides, Bifidobacterium, and Lactobacillus in the oral swab samples of the foreign students in South Asia was less than that in the domestic group (P0.05), and the number of E. coli and Enterococcus was much higher than that in the domestic group (P0.05). The difference was statistically significant. The quantitative comparison between the population of the oral cavity and the intestinal flora: the number of the total bacteria group (the oral swab sample) of the oral cavity group (the oral swab sample) is lower than that of the intestinal flora (stool sample), and the number of the Bacteroides, the genus Bifidobacterium, the genus Lactobacillus and the Escherichia coli is less (P0.05). The intestinal colonization resistance of the two groups was higher than that of the two groups (P0.05). The correlation between the number of Escherichia coli and the depression scale and the pressure scale in the stool sample was positively correlated (r = 0.243, P0.05; r = 0.217, P0.05); There was a positive correlation between the number of Escherichia coli and the depression scale in the oral swab samples (r = 0.250, P0.05). Conclusion 1. The foreign students in South Asia and the domestic students have good intestinal microecology, but the intestinal colonization resistance of the domestic students is stronger than that of the students in South Asia. The number of Bacteroides, Bifidobacterium, and Lactobacillus in the digestive tract of the international students in South Asia is less than that of the domestic students, while the number of Escherichia coli and Enterococcus is more than that of the domestic students. 3. The students in South Asia are in good health, but still have the depression and anxiety of individual students. There is a certain correlation between the number of Escherichia coli in the digestive tract and the psychological factors, but the specific mechanism of action is to be further studied.
【学位授予单位】：大理大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R57
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