炎症性肠病患者外周血IL-12家族细胞因子的表达及临床意义
发布时间：2019-03-18 10:00
【摘要】：炎症性肠病(IBD)是一组主要以肠道慢性炎症反应为表现的疾病,免疫异常被认为在疾病发病过程中具有非常重要的作用,其中细胞因子参与了肠道免疫反应与炎症进程。本研究通过检测对比98例IBD患者及24例肠息肉患者血清IL-12家族细胞因子含量,探讨其在炎症性肠病发病过程中的作用,为指导临床IBD的治疗提供新思路。目的探讨白介素12(IL-12)家族细胞因子包括IL-12、IL-23、IL-27及IL-35在炎症性肠病(inflammatory bowel disease)患者血清中的表达情况及其临床意义。方法经过数据质量处理,98例确诊为炎症性肠病(inflammatory bowel diseases,IBD)患者的外周血作为疾病组(其中克罗恩病61例,溃疡性结肠炎37例),另采集24例肠息肉患者的外周血作为对照组,利用酶联免疫吸附法(ELISA)检测疾病组与对照组血清中IL-12、IL-23、IL-27及IL-35的含量,比较各组细胞因子表达水平差异并分析其与疾病特征之间的关系。结果溃疡性结肠炎患者血清中IL-12、IL-23、IL-27的表达水平显著高于对照组(p值分别为0.001、0.001、0.023),IL-35的表达水平低于对照组(p值为0.001);克罗恩病患者血清中IL-12、IL-23、IL-27的表达水平均显著高于对照组,(p值分别为0.001、0.001、0.003),IL-35的表达水平低于对照组(p值为0.001);相关性分析显示IL-12、IL-23、IL-35血清表达水平与炎症性肠病(IBD)发病相关,(OR=1.2501,P=0.0407;OR=1.9554,P=0.0101;OR=0.92043,P=0.0139)。IL-12、IL-23及IL-35血清表达水平与溃疡性结肠炎的发病相关(OR=1.2490,P=0.0411;OR=1.9413,P=0.0124,OR=0.9159,P=0.0147),IL-12、IL-27及IL-35血清表达水平与克罗恩病的发病相关(OR=1.3964,P=0.0079;OR=1.0949,P=0.0469,OR=0.9408,P=0.0107);IL-12血清表达水平与克罗恩病以及溃疡性结肠炎病情的严重程度呈正相关(P0.001;P=0.0398),而其他细胞因子与克罗恩病、溃疡性结肠炎疾病严重程度间均无关联。结论IL-12家族细胞的血清表达均与炎症性肠病的发病关系密切,除IL-12外,其它细胞因子与炎症性肠病严重程度无显著关联。
[Abstract]:Inflammatory bowel disease (IBD) is a group of diseases characterized by chronic intestinal inflammation. Immune abnormalities are considered to play a very important role in the pathogenesis of inflammatory bowel diseases, in which cytokines play an important role in intestinal immune response and inflammation. In this study, the levels of serum IL-12 family cytokines in 98 patients with IBD and 24 patients with intestinal polyps were measured and compared to explore the role of cytokines in the pathogenesis of inflammatory bowel disease, and provide a new way to guide the treatment of clinical IBD. Objective to investigate the expression of interleukin-12 (IL-12) family cytokines including IL-12,IL-23,IL-27 and IL-35 in the serum of patients with inflammatory bowel disease (inflammatory bowel disease) and its clinical significance. Methods after data quality treatment, 98 patients with inflammatory bowel disease (inflammatory bowel diseases,IBD) were treated as the disease group (including 61 Crohn's disease and 37 ulcerative colitis), and all the patients were divided into two groups: Crohn's disease (61 cases) and ulcerative colitis (37 cases). The peripheral blood of 24 patients with intestinal polyps was collected as the control group. The levels of IL-12,IL-23,IL-27 and IL-35 in serum were detected by enzyme-linked immunosorbent assay (ELISA) in the patients with intestinal polyps and those in the control group. The expression level of cytokines in each group was compared and the relationship between cytokine expression and disease characteristics was analyzed. Results the expression of IL-12,IL-23,IL-27 in the serum of patients with ulcerative colitis was significantly higher than that in the control group (p = 0.001, 0.001, 0.023, respectively), and the expression level of IL-35 was lower than that in the control group (p = 0.001). The expression of IL-12,IL-23,IL-27 in serum of patients with Crohn's disease was significantly higher than that in control group (p = 0.001, 0.001, 0.003, respectively), and the expression level of IL-35 was lower than that of control group (p = 0.001). Correlation analysis showed that serum expression of IL-12,IL-23,IL-35 was associated with the pathogenesis of (IBD) in inflammatory bowel disease (OR=1.2501,P=0.0407;OR=1.9554,P=0.0101;). The expression of IL-12,IL-23 and IL-35 in serum was associated with the pathogenesis of ulcerative colitis (OR=1.2490,P=0.0411;). OR=0.92043,P=0.0139). The serum levels of OR=1.9413,P=0.0124,OR=0.9159,P=0.0147, IL-12,IL-27 and IL-35 were correlated with the pathogenesis of Crohn's disease (OR=1.3964,P=0.0079;OR=1.0949,P=0.0469,OR=0.9408,P=0.0107). The serum expression of IL-12 was positively correlated with the severity of Crohn's disease and ulcerative colitis (P0.001), but no correlation was found between other cytokines and the severity of Crohn's disease and ulcerative colitis. Conclusion the serum expression of IL-12 family cells is closely related to the pathogenesis of inflammatory bowel disease, except for IL-12, there is no significant correlation between other cytokines and the severity of inflammatory bowel disease.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R574.62
本文编号：2442751
[Abstract]:Inflammatory bowel disease (IBD) is a group of diseases characterized by chronic intestinal inflammation. Immune abnormalities are considered to play a very important role in the pathogenesis of inflammatory bowel diseases, in which cytokines play an important role in intestinal immune response and inflammation. In this study, the levels of serum IL-12 family cytokines in 98 patients with IBD and 24 patients with intestinal polyps were measured and compared to explore the role of cytokines in the pathogenesis of inflammatory bowel disease, and provide a new way to guide the treatment of clinical IBD. Objective to investigate the expression of interleukin-12 (IL-12) family cytokines including IL-12,IL-23,IL-27 and IL-35 in the serum of patients with inflammatory bowel disease (inflammatory bowel disease) and its clinical significance. Methods after data quality treatment, 98 patients with inflammatory bowel disease (inflammatory bowel diseases,IBD) were treated as the disease group (including 61 Crohn's disease and 37 ulcerative colitis), and all the patients were divided into two groups: Crohn's disease (61 cases) and ulcerative colitis (37 cases). The peripheral blood of 24 patients with intestinal polyps was collected as the control group. The levels of IL-12,IL-23,IL-27 and IL-35 in serum were detected by enzyme-linked immunosorbent assay (ELISA) in the patients with intestinal polyps and those in the control group. The expression level of cytokines in each group was compared and the relationship between cytokine expression and disease characteristics was analyzed. Results the expression of IL-12,IL-23,IL-27 in the serum of patients with ulcerative colitis was significantly higher than that in the control group (p = 0.001, 0.001, 0.023, respectively), and the expression level of IL-35 was lower than that in the control group (p = 0.001). The expression of IL-12,IL-23,IL-27 in serum of patients with Crohn's disease was significantly higher than that in control group (p = 0.001, 0.001, 0.003, respectively), and the expression level of IL-35 was lower than that of control group (p = 0.001). Correlation analysis showed that serum expression of IL-12,IL-23,IL-35 was associated with the pathogenesis of (IBD) in inflammatory bowel disease (OR=1.2501,P=0.0407;OR=1.9554,P=0.0101;). The expression of IL-12,IL-23 and IL-35 in serum was associated with the pathogenesis of ulcerative colitis (OR=1.2490,P=0.0411;). OR=0.92043,P=0.0139). The serum levels of OR=1.9413,P=0.0124,OR=0.9159,P=0.0147, IL-12,IL-27 and IL-35 were correlated with the pathogenesis of Crohn's disease (OR=1.3964,P=0.0079;OR=1.0949,P=0.0469,OR=0.9408,P=0.0107). The serum expression of IL-12 was positively correlated with the severity of Crohn's disease and ulcerative colitis (P0.001), but no correlation was found between other cytokines and the severity of Crohn's disease and ulcerative colitis. Conclusion the serum expression of IL-12 family cells is closely related to the pathogenesis of inflammatory bowel disease, except for IL-12, there is no significant correlation between other cytokines and the severity of inflammatory bowel disease.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R574.62
【参考文献】
相关期刊论文 前1条
1 白爱平;;炎症性肠病发病机制的微生物因素[J];世界华人消化杂志;2006年07期
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