侧脑室微量注射ghrelin对大鼠小肠转运的作用及其机制

发布时间：2019-03-20 15:33

【摘要】：目的探讨侧脑室微量注射ghrelin对大鼠小肠运动的作用及对中枢和胃肠道c-Fos表达的影响。方法 1给大鼠分别埋置十二指肠导管及侧脑室套管,禁食24h后经侧脑室微量注射生理盐水或ghrelin(0.4、1.6μg/kg或6.4μg/kg),经十二指肠导管注射伊文氏蓝溶液,观察不同剂量ghrelin对小肠转运的影响。另2组大鼠分别接受侧脑室注射ghrelin受体拮抗剂(D-Lys3)GHRP-6(3.7μg/kg)、(D-Lys3)GHRP-6(3.7μg/kg)+ghrelin(6.4μg/kg),探讨ghrelin对小肠转运的作用机制。2采用免疫组织化学和图像分析方法观察侧脑室注射ghrelin对大鼠中枢神经系统和肠神经系统c-Fos蛋白表达的影响,并观察(D-Lys3)GHRP-6对ghrelin作用的影响。结果 1侧脑室微量注射ghrelin 0.4μg/kg对大鼠小肠转运无显著影响,ghrelin 1.6μg/kg和6.4μg/kg可促进小肠转运,此作用被(D-Lys3)GHRP-6阻断。2侧脑室微量注射ghrelin可激活中枢多个部位的c-Fos蛋白表达,包括下丘脑弓状核、室旁核、下丘脑腹内侧核、杏仁内侧核、迷走神经背核等核团;胃、十二指肠、近端结肠的c-Fos表达增加,胃的c-Fos蛋白表达增加最显著。Ghrelin受体拮抗剂(D-Lys3)GHRP-6可抑制ghrelin激活的中枢神经系统和肠神经系统的c-Fos蛋白表达。结论侧脑室微量注射ghrelin促进大鼠小肠转运,其促动力作用可能通过受体GHSR介导。侧脑室微量注射ghrelin可通过中枢神经系统和肠神经系统调节小肠运动。
[Abstract]:Objective To study the effect of the microinjection of ghrelin on the small bowel movement in rats and the effect on the expression of c-Fos in the central and gastrointestinal tract. Method 1 The rats were respectively embedded in the duodenum and the lateral ventricles of the lateral ventricle. After fasting for 24 h, the rats were injected with saline or ghrelin (0.4, 1.6. mu.g/ kg or 6.4. mu.g/ kg) in the lateral ventricle, and the Evan's blue solution was injected through the duodenal tube, and the effect of the different dose of ghrelin on the small intestine was observed. Another group of rats received the intraventricular injection of the ghrelin receptor antagonist (D-Lys3) GHRP-6 (3.7. mu.g/ kg), (D-Lys3) GHRP-6 (3.7. mu.g/ kg) + ghrelin (6.4. mu.g/ kg), The effect of ghrelin on the expression of c-Fos protein in the central nervous system and the intestinal system of rats was studied by immunohistochemistry and image analysis, and the effect of (D-Lys3) GHRP-6 on the effect of ghrelin was observed. Results The intraventricular microinjection of ghrelin 0.4. mu.g/ kg had no significant effect on the small intestinal transit of rats, and ghrelin 1.6. mu.g/ kg and 6.4. m u.g/ kg could promote the small intestinal transport, and the effect was blocked by the (D-Lys3) GHRP-6. The c-Fos protein expression in the central multiple sites was activated by the microinjection of ghrelin in the lateral ventricle, including the hypothalamic arcuate nucleus and the paraventricular nucleus. The expression of c-Fos in the stomach, duodenum and proximal colon was increased, and the expression of c-Fos protein in the stomach was most significant. Ghrelin receptor antagonist (D-Lys3) GHRP-6 can inhibit the expression of c-Fos protein in the central nervous system and the intestinal nervous system activated by ghrelin. Conclusion The microinjection of ghrelin in the lateral ventricle promotes the small intestinal transport in the rat, and its prokinetic action may be mediated by the receptor GHSR. The microinjection of ghrelin in the lateral ventricle can regulate the small intestinal movement through the central nervous system and the intestinal nervous system.
【作者单位】：西安交通大学医学部第二附属医院消化内科陕西省肠胃动力疾病研究重点实验室陕西省胃肠疾病临床研究中心;
【基金】：国家自然科学基金资助项目(No.81100258;81200310) 陕西省社会发展攻关计划资助项目(No.2015SF102)~~
【分类号】：R57

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