盐酸小檗碱对高脂饮食诱导的非酒精性脂肪性肝病大鼠肠道菌群的影响

发布时间：2019-04-07 19:20

【摘要】：目的观察盐酸小檗碱对高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)大鼠肠道菌群的影响。方法 18只雄性SD大鼠随机分为普通饲料喂养组(正常对照组)、高脂饮食诱导NAFLD组(NAFLD组)和高脂饮食诱导NAFLD小檗碱干预组(小檗碱干预组),每组6只。采用16SrRNA序列分析法研究3组大鼠肠道菌群的变化;观察3组大鼠肝脏和回肠组织形态学改变。结果 3组大鼠的肠道菌群主要分布在拟杆菌门和厚壁菌门;与正常对照组比较,NAFLD组的厚壁菌门比例显著增高(P0.05),拟杆菌门比例及有益菌乳酸菌属比例则显著降低(P0.01);与NAFLD组比较,小檗碱干预组的厚壁菌门比例显著下降(P0.01),拟杆菌门比例和有益菌乳酸菌属比例显著增高(P0.01)。肝脏组织学检查发现:正常对照组大鼠肝脏细胞排列正常,未见脂肪滴和炎症细胞浸润;NAFLD组大鼠肝脏组织出现大量的脂肪变性,肝细胞排列较正常对照组紊乱;小檗碱干预组大鼠肝脏组织仍有脂肪变性,但脂肪变性程度较NAFLD组减轻。回肠组织学检查发现:正常对照组大鼠回肠绒毛排列整齐、紧凑,表面结构完整;NAFLD组大鼠回肠绒毛出现断裂、缺失,间隙增宽;小檗碱干预组大鼠回肠绒毛排列较为整齐,断裂、缺失较NAFLD组少。结论小檗碱可以改善高脂饮食所诱导的NAFLD,其机制可能与改变肠道菌群的结构及减轻高脂饮食导致的肠道黏膜损伤有关。
[Abstract]:Aim to observe the effect of berberine hydrochloride on intestinal flora in (NAFLD) rats with nonalcoholic fatty liver disease induced by high fat diet. Methods 18 male SD rats were randomly divided into three groups: normal diet group (normal control group), high fat diet induced NAFLD group (NAFLD group) and high fat diet induced NAFLD berberine intervention group (berberine intervention group), with 6 rats in each group. 16SrRNA sequence analysis was used to study the changes of intestinal microflora and histological changes of liver and ileum in three groups. Results the intestinal microflora of the three groups of rats was mainly distributed in the myxomycetes and the thick-walled fungi. Compared with the normal control group, the proportion of thick-walled bacteria in NAFLD group was significantly higher (P0.05), while the proportion of mycorrhizal bacteria and the proportion of beneficial lactic acid bacteria were significantly decreased (P0.01). Compared with NAFLD group, the percentage of thick-walled bacteria in berberine intervention group decreased significantly (P0.01), and the proportion of mycorrhizal bacteria and the proportion of lactic acid bacteria of beneficial bacteria increased significantly in berberine intervention group (P0.01). Liver histological examination showed that normal liver cells were arranged in normal control group, fat droplets and inflammatory cells were not seen in NAFLD group, fatty degeneration appeared in liver tissue of normal control group, hepatic cell arrangement was disordered compared with normal control group, and there was no fat drop and inflammatory cell infiltration in liver tissue of normal control group. Steatosis was found in the liver tissue of the berberine intervention group, but the degree of steatosis was less than that of the NAFLD group. The histological examination of the ileum showed that the villi of the ileum of the normal control group were arranged orderly, compact and the surface structure was intact, and the villi of the ileum of the NAFLD group were broken, missing and the gap widened. The villi of ileum of berberine intervention group were neatly arranged and broken, and the deletion was less than that of NAFLD group. Conclusion berberine can improve NAFLD, induced by high fat diet and its mechanism may be related to changing the structure of intestinal flora and reducing intestinal mucosal damage induced by high fat diet.
【作者单位】：上海交通大学医学院附属第九人民医院内分泌科;
【基金】：国家重点基础研究发展计划(“973”计划)(2012CB524906)~~
【分类号】：R575.5

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