TNF-α抑制剂在细胞水平上抑制丙型肝炎病毒的感染

发布时间：2019-04-17 06:51

【摘要】：目的:探索TNF-α抑制剂对丙型肝炎病毒感染肝细胞的影响。方法:体外培养肝癌细胞株Huh7细胞,分为未处理组、NFκB抑制剂[6-amino-4-(4-phenoxyphenylethylamino)quinazoline,QNZ]处理组、咖啡酸苯乙酯(caffeic acid phenethyl ester,CAPE)处理组和TNF-α抑制剂来那度胺(Lenalidomide)处理组,每组设置浓度梯度为100μmol/L至1.28 nmol/L,5倍倍比稀释,通过CCK8法检测各抑制剂对细胞增殖的影响;Huh7细胞经抑制剂处理后,再以丙型肝炎病毒感染,通过荧光定量PCR和免疫荧光法检测细胞内病毒的感染水平。结果:100μmol/L QNZ处理组(0.730±0.056)和100μmol/L CAPE处理组(1.040±0.014)细胞在450 nm处的吸光度(absorbance,A)值均低于未处理组(1.190±0.040,P=0.000)。Lenalidomide各浓度处理组(100μmol/L处理组,1.250±0.016)细胞增殖水平与未处理组相当(P=0.306)。在抗病毒活性方面,100 nmol/L Lenalidomide处理组[(3.79±0.88)×105]和QNZ处理组[(6.64±1.11)×105]HCV RNA水平明显低于未处理组[(1.51±0.45)×10~6,P=0.000],而CAPE处理组[(1.61±0.15)×10~6]与未处理组HCV RNA水平相当(P=0.383)。结论:TNF-α抑制剂Lenalidomide对细胞毒性较小,能够在细胞水平上抑制丙型肝炎病毒的感染。
[Abstract]:Objective: to explore the effect of TNF- 伪 inhibitor on hepatitis C virus (HCV) infected hepatocytes. Methods: hepatocellular carcinoma cell line Huh7 was cultured in vitro and divided into untreated group, NF 魏 B inhibitor [6-(4-phenoxyphenylethylamino) quinazoline,QNZ] group, caffeic acid phenethyl ester (caffeic acid phenethyl ester, group. CAPE) treatment group and TNF- 伪 inhibitor lenalidomide (Lenalidomide) treatment group, the concentration gradient of each group was 100 渭 mol / L to 1.28 nmol/L,5 times dilution, and the effect of each inhibitor on cell proliferation was detected by CCK8 method. Huh7 cells were treated with inhibitors and then infected with hepatitis C virus (HCV). The intracellular viral infection level was detected by fluorescence quantitative PCR and immunofluorescence assay. Results: the absorbance (absorbance,A) of the cells treated with 100 渭 mol / L QNZ (0.730 卤0.056) and 100 渭 mol / L CAPE (1.040 卤0.014) at 450 nm was lower than that of the untreated group (1.190 卤0.040). The proliferation level of Lenalidomide treated group (100 渭 mol / L, 1.250 卤0.016) was similar to that of non-treated group (P < 0. 306). In terms of antiviral activity, the level of HCV RNA in nmol/L Lenalidomide treatment group [(3.79 卤0.88) 脳 10 ~ 5] and QNZ treatment group [(6.64 卤1.11) 脳 10 ~ 5] was significantly lower than that in untreated group [(1.51 卤0.45) 脳 10 脳 10 ~ 6, P < 0.001]. The level of HCV RNA in CAPE treated group [(1.61 卤0.15) 脳 10 脳 10 6] was similar to that in untreated group (P = 0.383). Conclusion: Lenalidomide, an inhibitor of TNF- 伪, can inhibit hepatitis C virus infection at cell level.
【作者单位】： 成都军区总医院检验科;成都军区疾病预防控制中心;
【基金】：国家自然科学基金资助项目(编号:81301445) 院管课题基金资助项目(编号:2013YG-B055)
【分类号】：R512.63
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