小鼠胃肠动力分型障碍模型探索研究

发布时间：2019-04-18 18:58

【摘要】：目的:初步探索小鼠胃肠动力分型障碍模型。方法:对正常小鼠胃肠动力分析,以小鼠胃排空率和小肠推进率为指标分别建立小鼠胃肠动力评价标准,将小鼠胃肠动力分为抑制、稳定和亢进三种类型。采用综合造模法建立小鼠胃肠动力障碍模型,分别于造模2、4、6、8、10 d后处死小鼠,测定小鼠胃残留率和小肠推进率,确定小鼠胃肠动力障碍模型的造模时间,选择适合实验的小鼠胃肠动力模型评价指标。结果:正常小鼠胃肠动力标准建立:以胃残留率为标准建立模型评价,小鼠胃残留率在(60.00±15.86)%为稳定型,胃残留率在(98.39±2.71)%为抑制型,胃残留率在(21.86±9.13)%为亢进型;以小肠推进率为标准建立模型评价,小鼠胃残留率在(56.93±21.89)%为稳定型,胃残留率在(8.48±7.20)%为抑制型,胃残留率在(97.30±3.12)%为亢进型。建立小鼠胃肠动力障碍模型,以第2、4、6天作为小鼠胃肠动力障碍模型的造模时间,以小肠推进率为标准建立的模型评价作为小鼠胃肠动力障碍造模方法的评价方法。结论:正常小鼠中存在胃肠动力障碍问题,以胃残留率和小肠推进率为标准分别建立小鼠胃肠动力障碍模型评价标准,以小肠推进率为标准建立的模型评价作为小鼠胃肠动力障碍造模方法的评价方法。
[Abstract]:Objective: to explore the model of gastrointestinal motility disorder in mice. Methods: the gastrointestinal motility of normal mice was analyzed. The evaluation criteria of gastrointestinal motility were established based on the gastric emptying rate and small intestinal propulsive rate of mice. The gastrointestinal motility of mice was divided into three types: inhibition, stability and hyperactivity. The model of gastrointestinal motility disorder in mice was established by comprehensive modeling method. The mice were killed after 2,4,6,8 days, respectively. The gastric remnant rate and small intestinal propulsion rate of mice were measured, and the time of establishment of gastrointestinal motility disorder model was determined, and the time of establishment of gastrointestinal motility disorder model was determined by measuring the gastric remnant rate and small intestinal propulsive rate of mice. The evaluation indexes of gastrointestinal motility model in mice were selected. Results: the standard of gastrointestinal motility in normal mice was established. The gastric remnant rate was (60.00 卤15.86)% as stable type and (98.39 卤2.71)% as inhibitory type, and the gastric remnant rate was evaluated by using gastric remnant rate as standard, and the gastric residual rate was (98.39 卤2.71)% as inhibitory type. The gastric residual rate was (21.86 卤9.13)%. The gastric remnant rate was (56.93 卤21.89)%, the gastric residual rate was (8.48 卤7.20)%, and the gastric residual rate was (97.30 卤3.12)%, the gastric remnant rate was (97.30 卤3.12)%, which was the stable type, the inhibitory type was (8.48 卤7.20)%, and the gastric residual rate was (97.30 卤3.12)%. The model of gastrointestinal motility disorder in mice was established. The time of establishment of the model was taken as the time of establishment of the model of gastrointestinal motility disorder in mice on the 2nd, 4th and 6th day, and the evaluation of the model established according to the standard of small intestinal propulsive rate was used to evaluate the method of establishing the model of gastrointestinal motility disorder in mice. Conclusion: there are gastrointestinal motility disorders in normal mice. The evaluation criteria of gastrointestinal motility disorders in mice were established based on the gastric remnant rate and the small intestinal propulsive rate, respectively. The model evaluation based on the small intestine propulsive rate was used to evaluate the model of gastrointestinal motility disorder in mice.
【作者单位】：成都中医药大学;四川好医生药业集团有限公司;
【基金】：国家自然科学基金(30973713)
【分类号】：R57;R-332

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