应用Forley导尿管建立兔反流性食管炎模型

发布时间：2019-04-18 23:08

【摘要】：目的探讨一种更安全有效且符合生理解剖的反流性食管炎动物模型的建立方法。方法健康5个月龄新西兰大白兔45只,随机均分为3组:贲门括约肌切开术组(A组),贲门括约肌部分切除术组(B组),Forley导尿管气囊扩张术组(C组)。建模前行食管造瘘术,3组兔分别采用各自方法建立反流性食管炎模型,术前及术后2周进行24hpH值监测,术后4周处死动物,观察大体标本及镜下标本。结果 B组死亡率较其余两组高(P=0.057)。B组兔24h内pH4的时间百分比、反流次数及pH4持续时间超过5min的反流次数均高于其余两组,C组略高于A组,但差异无统计学意义;三组造模成功率几近相同。造模成功的兔中,B组中重度反流性食管炎比例较高,但差异无统计学意义。结论贲门括约肌部分切除术造模效果较为理想,但死亡率高;气囊扩张术术式简单,安全性高,造模效果理想,且符合正常生理解剖结构,适合内镜治疗及其他新型治疗方法的基础研究。
[Abstract]:Objective to establish a safe and effective animal model of reflux esophagitis. Methods 45 New Zealand white rabbits aged 5 months were randomly divided into three groups: cardiac sphincterotomy group (group A), cardiac sphincter partial resection group (group B), Forley catheter balloon dilation group (group C). Three groups of rabbits were used to establish reflux esophagitis model respectively. The pH value was monitored 24 hours before and 2 weeks after operation. The animals were killed at 4 weeks after operation to observe the gross and microscopic specimens. Results the mortality rate of group B was higher than that of the other two groups (P < 0.05). The percentage of pH4 time within 24 hours, the number of reflux times and the duration of pH4 over 5min in group B were higher than those in the other two groups, and the mortality rate in group C was slightly higher than that in group A, but there was no significant difference between group C and group A. The success rate of the three groups is almost the same. The rate of moderate to severe reflux esophagitis in group B was higher than that in group B, but there was no significant difference between the two groups. Conclusion partial gastrectomy of cardiac sphincter is effective, but the mortality is high. Balloon dilation has the advantages of simple, high safety, ideal effect of modeling, and accord with normal physiological and anatomical structure. It is suitable for the basic research of endoscopic therapy and other new treatment methods.
【作者单位】：第二军医大学长征医院普外三科;
【基金】：上海市卫生和计划生育委员会科研课题青年项目(20144Y0199)~~
【分类号】：R571;R-332

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