非酒精性脂肪肝病与肝细胞癌关系的研究
发布时间：2019-05-30 09:37
【摘要】：目的 :研究非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)与肝细胞癌(hepatocellular carcinoma,HCC)的关系,并探讨核因子κB(nuclear factor-κB,NF-κB)以及白细胞介素6(interleukin-6,IL-6)在致病机制中的作用。方法:将小鼠分为空白对照组、常规饮食组及高脂饮食组,从第16周起至36周对高脂饮食组及常规饮食组小鼠予以二乙基亚硝胺(diethylnitrosamine,DEN)45 mg/kg每周1次腹腔注射,诱导小鼠形成HCC,空白对照组不予任何药物干预;于第15、20、25和30周在常规饮食组及高脂饮食组中各取5只小鼠,空白对照组取3只小鼠,收集血清及肝组织;第36周处死所有小鼠,同样收集小鼠血清及肝组织,并行HE染色分析肝组织形态学的变化,比较各组小鼠癌变率的差异,动态监测各组小鼠不同时期血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、甘油三酯(triglyceride,TG)和总胆固醇(total cholesterol,TC)的表达情况;ELISA法检测血清中NF-κB和IL-6的含量。结果 :喂养15周后高脂饮食组小鼠成功建立NAFLD模型,空白对照组小鼠至第36周无自发成癌现象出现。常规饮食组小鼠诱癌成功率为40.0%(8/20),高脂饮食组小鼠诱癌成功率为83.3%(13/18)。各时期小鼠血清NF-κB和IL-6在高脂饮食组小鼠中的表达水平均明显高于常规饮食组(P值均0.01)。结论 :NAFLD能促进DEN对肝脏的致癌作用。炎性反应因子NF-κB和IL-6作为促癌因子,其异常表达在NAFLD向HCC的进展过程中发挥着重要的作用。
[Abstract]:Objective: to study the relationship between nonalcoholic fatty liver disease (non-alcoholic fatty liver disease,NAFLD) and hepatocellular carcinoma (hepatocellular carcinoma,HCC), and to explore the relationship between nuclear factor kappa B (nuclear factor- (NF-) and interleukin 6 (interleukin-6,). IL-6) plays an important role in pathogenesis. Methods: the mice were divided into blank control group, routine diet group and high-fat diet group. The mice in the high-fat diet group and the conventional diet group were given intraabdominal injection of diethylnitrosamine (diethylnitrosamine,DEN) 45 mg/kg once a week from the 16th week to the 36th week. The blank control group induced HCC, in mice was not interfered with any drugs. At the 15th, 20th, 25th and 30th week, 5 mice were taken in the routine diet group and 5 mice in the high fat diet group, and 3 mice in the blank control group were taken to collect serum and liver tissue. At the 36th week, all the mice were killed. The serum and liver tissue of the mice were also collected. The morphological changes of liver tissue were analyzed by HE staining. The difference of cancer rate in each group was compared. The serum glutamic-pyruvic transaminase (alanine aminotransferase, in different periods of each group was dynamically monitored. The expression of glutamic oxaloacetic transaminase (aspartate aminotransferase,AST), triglyceride (triglyceride,TG) and total cholesterol (total cholesterol,TC) in ALT),. The contents of NF- 魏 B and IL-6 in serum were detected by ELISA. Results: the NAFLD model was successfully established in the high fat diet group after 15 weeks of feeding, and no spontaneous carcinogenesis occurred in the blank control group at the 36th week. The success rate of cancer induction in conventional diet group was 40.0% (8 鈮，
本文编号：2488736
[Abstract]:Objective: to study the relationship between nonalcoholic fatty liver disease (non-alcoholic fatty liver disease,NAFLD) and hepatocellular carcinoma (hepatocellular carcinoma,HCC), and to explore the relationship between nuclear factor kappa B (nuclear factor- (NF-) and interleukin 6 (interleukin-6,). IL-6) plays an important role in pathogenesis. Methods: the mice were divided into blank control group, routine diet group and high-fat diet group. The mice in the high-fat diet group and the conventional diet group were given intraabdominal injection of diethylnitrosamine (diethylnitrosamine,DEN) 45 mg/kg once a week from the 16th week to the 36th week. The blank control group induced HCC, in mice was not interfered with any drugs. At the 15th, 20th, 25th and 30th week, 5 mice were taken in the routine diet group and 5 mice in the high fat diet group, and 3 mice in the blank control group were taken to collect serum and liver tissue. At the 36th week, all the mice were killed. The serum and liver tissue of the mice were also collected. The morphological changes of liver tissue were analyzed by HE staining. The difference of cancer rate in each group was compared. The serum glutamic-pyruvic transaminase (alanine aminotransferase, in different periods of each group was dynamically monitored. The expression of glutamic oxaloacetic transaminase (aspartate aminotransferase,AST), triglyceride (triglyceride,TG) and total cholesterol (total cholesterol,TC) in ALT),. The contents of NF- 魏 B and IL-6 in serum were detected by ELISA. Results: the NAFLD model was successfully established in the high fat diet group after 15 weeks of feeding, and no spontaneous carcinogenesis occurred in the blank control group at the 36th week. The success rate of cancer induction in conventional diet group was 40.0% (8 鈮，
本文编号：2488736
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