二氢杨梅素对小鼠葡聚糖硫酸钠结肠炎的疗效

发布时间：2019-06-12 17:22

【摘要】：目的观察二氢杨梅素(dihydromyricetin,DHM)治疗溃疡性结肠炎(ulcerative colitis,UC)的效果并探讨其机制。方法 60只BALB/c小鼠采用随机数字表法分为正常组,模型组,DHM低剂量[50 mg/(kg·d)]、中剂量[100 mg/(kg·d)]、高剂量[200 mg/(kg·d)]组,5-氨基水杨酸[5-amino saliciylic acid,5-ASA,50 mg/(kg·d)]组。分组当日给予正常组小鼠蒸馏水自行饮用,其余组小鼠给予4%葡聚糖硫酸钠(DSS)水溶液7 d诱导急性UC模型。于饮用DSS当日开始,用DHM各剂量组及5-氨基水杨酸组按各组拟定剂量分别进行灌胃处理,连续给药14 d。对比各组疾病活动指数(DAI)、结肠长度、脾脏指数的变化、结肠组织学评分,肠组织TLR2、TLR4和NF-κB的活性以及炎性细胞因子IL-1β、TNF-α、IL-6表达及MPO、NO、SOD的活性变化。结果与模型组比较,DHM可显著降低结肠组织中TLR2、TLR4、NF-κB的表达,减少炎性因子如IL-1β、TNF-α、IL-6水平,同时还能够减少肠组织中MPO、NO活性及增加SOD的活性。其中DHM高剂量组优于低、中剂量组,差异有统计学意义(P0.05),治疗效果最明显,其与5-氨基水杨酸组相比差异无统计学意义(P0.05),治疗效果相当。结论 DHM可显著缓解DSS诱导的小鼠结肠炎症,其作用机制可能与抗炎、抗氧化有关。
[Abstract]:Objective To observe the effect of dihydromyricetin (DHM) on ulcerative colitis (UC) and its mechanism. Methods 60 BALB/ c mice were divided into normal group, model group, DHM low dose[50 mg/ (kg 路 d)], middle dose[100 mg/ (kg 路 d)], high dose[200 mg/ (kg 路 d)] and 5-aminosalicylic acid[5-amino salicylic acid,5-ASA,50 mg/ (kg 路 d)]. The normal group of mice were given the normal group of distilled water for self-drinking on the same day, and the rest of the mice were given 4% sodium dextran sulfate (DSS) aqueous solution for 7 days to induce the acute UC model. At the beginning of the day of drinking, the dosage groups of DHM and 5-aminosalicylic acid were administered intragastric respectively for 14 days. The changes of the activity index (DAI), the length of the colon, the index of the spleen, the histological grade of the colon, and the intestinal tissue TLR2 were compared. The activity of TLR4 and NF-EMAB and the activity of IL-1, TNF-1, IL-6 and MPO, NO, and SOD of inflammatory cytokines. Results Compared with the model group, DHM can significantly lower the expression of TLR2, TLR4, NF-B in the colon tissue, reduce the level of the inflammatory factors such as IL-1, TNF-1, and IL-6, and also reduce the activity of MPO and NO in the intestinal tissue and increase the activity of SOD. The results showed that the high dose group of DHM was superior to that of the low and middle dose group (P0.05). The treatment effect was the most obvious. The difference of the treatment effect was not significant (P0.05), and the treatment effect was comparable. Conclusion DHM can alleviate the colonic inflammation of mice induced by DSS, and its mechanism of action may be related to anti-inflammatory and anti-oxidation.
【作者单位】：兰州大学第二医院消化科;甘肃省人民医院西院消化科;甘肃省消化系肿瘤重点实验室;
【基金】：国家自然科学基金面上项目(81272661) 甘肃省自然科学基金(1506RJZA204)~~
【分类号】：R574.62

【相似文献】