烟碱及单侧迷走神经切断对重症急性胰腺炎模型大鼠肺损伤的影响

发布时间：2019-07-26 07:52

【摘要】：目的观察烟碱和迷走神经切断对大鼠重症急性胰腺炎(SAP)肺损伤的影响。方法 SPF级雌性Wistar大鼠48只,按随机数字表法分为6组,8只/组,分别予SAP造模假手术+生理盐水治疗(Sham+saline组),SAP+生理盐水治疗(SAP+saline组),SAP+烟碱治疗(SAP+nicotine组);正常对照大鼠+迷走神经切断假手术(Con+sham surgery组),SAP+迷走神经切断假手术(SAP+sham surgery组),SAP+迷走神经切断(SAP+vagotomy组)。采用胆胰管逆行注射5%牛磺胆酸钠建立大鼠SAP模型,术前30 min腹腔注射烟碱,或术前3 d行左侧迷走神经切断。术后12 h剖杀大鼠,观察胰腺、肺组织病理学变化,检测血清淀粉酶(AMY)、脂肪酶(LIPA),酶联免疫吸附法(ELISA)检测血清白细胞介素-6(IL-6),试剂盒测定肺组织髓过氧化物酶(MPO),免疫印迹法(Western blot)检测肺组织高迁移率族蛋白B1(HMGB1)。结果 SAP+saline组胰腺、肺组织病理学评分,AMY、LIPA、IL-6、MPO和HMGB1均较Sham+saline组升高(P0.05),SAP+nicotine组上述指标均较SAP+saline组下降(P0.05),SAP+sham surgery组上述指标均较Con+sham surgery组升高(P0.05),SAP+vagotomy组上述指标均较SAP+sham surgery组升高(P0.05)。结论烟碱可通过下调IL-6、MPO和HMGB1减轻SAP肺损伤,单侧迷走神经切断可加剧SAP肺损伤。
[Abstract]:Objective to observe the effects of nicotine and vagus nerve transection on (SAP) lung injury in rats with severe acute pancreatitis (SAP). Methods 48 SPF female Wistar rats were randomly divided into 6 groups, 8 rats / group, treated with), SAP saline in (Sham saline group and), SAP nicotine group in (SAP saline group, respectively, and normal control rats were treated with), SAP vagus nerve transection in (Con sham surgery group and), SAP vagus nerve transection (SAP vagotomy group in (SAP sham surgery group. The rat model of SAP was established by retrograde injection of 5% sodium taurocholate into the cholancreatic duct. Nicotine was injected intraperitoneally 30 min before operation or left vagus nerve was cut off 3 days before operation. Rats were killed 12 hours after operation. Pathological changes of pancreas and lung tissue were observed, serum amylase (AMY), lipase (LIPA), enzyme linked immunosorbent assay (Elisa) (ELISA) was used to detect serum IL-6 (IL-6), and lung tissue myeloperoxidase (MPO), immunoblotting (Western blot) was used to detect high mobility group protein B1 (HMGB1) in lung tissue. Results the pathological scores of pancreas and lung tissue, AMY,LIPA,IL-6,MPO and HMGB1 in SAP saline group were higher than those in Sham saline group (P 0.05), and the above indexes in), SAP nicotine group were lower than those in SAP saline group (P 0.05). The above indexes in), SAP sham surgery group were higher than those in Con sham surgery group (P 0.05), and the above indexes in), SAP vagotomy group were higher than those in SAP sham surgery group (P 0.05). Conclusion nicotine can reduce lung injury in SAP by down-regulating IL-6,MPO and HMGB1, and unilateral vagotomy can aggravate lung injury in SAP.
【作者单位】：南方医科大学附属南海医院消化内科;
【基金】：广东省佛山市医学类科技攻关项目(编号:2014AB000132)
【分类号】：R576;R-332

【相似文献】