FTY720对实验性肝纤维化小鼠肝损伤的保护作用

发布时间：2019-08-19 08:35

【摘要】：目的:观察FTY720对刀豆蛋白A(Con A)所致肝纤维化小鼠的保护作用,探讨其可能的作用机制。方法:采用刀豆蛋白A(Con A)建立小鼠肝纤维化动物模型,取40只BALB/C小鼠随机分为空白对照组、模型组、FTY720低剂量(1mg·kg-1)组和FTY720高剂量(4mg·kg-1)组,每组10只。测定各组小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性和肝脏指数,观察肝脏组织学病理变化。结果:FTY720低剂量组和高剂量组ALT、AST活性明显低于模型组(P0.05或P0.01)。光学显微镜观察,模型组有炎性细胞浸润,肝细胞坏死。Masson染色,模型组血管周围有明显纤维条束,肝小叶发生融合;与模型组比较,FTY720低剂量组和高剂量组损伤程度较轻,且随FTY720剂量增加肝组织损伤程度逐渐减轻。结论:FTY720对实验性肝纤维化小鼠的保护作用可能是通过降低ALT和AST活性,进而减轻肝脏损伤程度而延缓纤维化进程。
[Abstract]:Aim: to observe the protective effect of FTY720 on liver fibrosis induced by concanavalin A (Con A) in mice and to explore its possible mechanism. Methods: the animal model of liver fibrosis in mice was established by concanavalin A (Con A). 40 BALB/C mice were randomly divided into blank control group, model group, FTY720 low dose (1mg kg-1) group and FTY720 high dose (4mg kg-1) group with 10 mice in each group. The activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver index were measured in each group, and the pathological changes of liver were observed. Results: the activity of ALT,AST in FTY720 low dose group and high dose group was significantly lower than that in model group (P 0.05 or P 0.01). Compared with the model group, the injury degree of FTY720 low dose group and high dose group was less than that of the model group, and the degree of liver tissue injury was gradually alleviated with the increase of FTY720 dose. Masson staining showed that there were obvious fiber bundles around the blood vessels and fusion of hepatic lobule around the blood vessels in the model group. Compared with the model group, the injury degree of liver tissue in the low dose group and the high dose group was less than that in the model group. Conclusion: the protective effect of FTY720 on experimental hepatic fibrosis in mice may be to delay the process of fibrosis by reducing the activities of ALT and AST, and then reducing the degree of liver injury.
【作者单位】：吉林医药学院检验学院病原生物学教研室;
【基金】：国家自然科学基金资助课题(82102953) 吉林省科技厅中青年科技创新领军人才及团队资助课题(20130521018JH);吉林省科技厅重点科技攻关项目资助课题(20140203012YY) 吉林省教育厅“十二五”科学技术研究项目资助课题(2012第487号) 吉林省卫生厅重点专科项目资助课题(2013Z091)
【分类号】：R575.2

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