IL-9及TL1A在溃疡性结肠炎患者肠黏膜组织中的表达变化
发布时间：2020-07-13 03:51
【摘要】：溃疡性结肠炎(ulcerative colitis,UC)发病机制不明,多数学者认为免疫因素是其主要发病机制。Th9细胞是一种新发现的CD4~+T细胞亚群,主要分泌白细胞介素9(Interleukin-9,IL-9),PU.1是其特异性转录因子。研究证实,Th9细胞及IL-9参与UC发生发展,并成为研究热点。肿瘤坏死因子样配体1A(tumor necrosis factor ligand-related molecule1A,TL1A)是TNFSF15编码的蛋白产物,GWAS研究证实TNFSF15是UC的易感基因。研究发现UC患者中TL1A表达明显升高,并与肠道炎症程度呈正相关。TL1A过表达慢性实验性结肠炎模型中,TL1A与其受体肿瘤坏死因子受体超家族成员死亡受体3(death receptor 3,DR3)结合,通过PU.1促进Th9分化、IL-9分泌,从而参与UC进程,并与肠道炎症程度呈正相关。多项研究提示IL-9及TL1A在UC发病机制中发挥重要作用。目的:探讨IL-9及TL1A在UC患者血清及肠组织中的表达,以期为UC寻求新的治疗方法。方法:ELISA检测血清TL1A、IL-9含量,IF检测肠组织Th9细胞含量,IHC检测肠组织TL1A、DR3、PU.1含量,Real-time PCR检测肠组织TL1A、DR3、PU.1、IL-9mRNA含量。结果:(1)血清中IL-9与TL1A呈正相关。其含量均随疾病活动度、内镜下Mayo评分、病变累及范围增加其含量增加,除E1组与E2组相比无统计学意义外(p0.05),其余两两相比均有统计学意义(p0.05)。(2)肠组织中Th9、IL-9、PU.1、DR3及TL1A含量均随疾病活动度、内镜下Mayo评分、病变累及范围增加其含量增加,除E1组与E2组相比无统计学意义外(p0.05),其余两两相比均有统计学意义(p0.05),并验证了Th9细胞、IL-9与TL1A呈正相关,PU.1与IL-9、Th9呈正相关,DR3与TL1A呈正相关。结论:TL1A、DR3、Th9细胞、PU.1、IL-9参与UC的发生发展,并推测TL1A与DR3结合后可能通过调控PU.1促进了Th9细胞分化及IL-9的分泌,从而参与UC的进程。
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2018
【分类号】：R574.62
【图文】：
a. normal mucosal; b. hyperemia and punctate erythema; c. mucosal edema,flaky erythema; d. ulcers, blooding and purulent discharge图2 溃疡性结肠炎肠黏膜组织病理改变Fig.2 Pathological changes of colon tissue: H&E staining (×400)a.normal mucosal; b.mild inflammatory changes in intrinsic membrane diffusethe impatient chronic inflammatory cell infiltration and a crypt abscess,c.moderate inflammatory change:crypt structure change;d.severe inflammatorychanges: mucosal surface erosion, shallow ulceration and granulation tissueformation
图 1 溃疡性结肠炎内镜下 Mayo 评分Fig.1 Different performance of colonoscopya. normal mucosal; b. hyperemia and punctate erythema; c. mucosal edema,flaky erythema; d. ulcers, blooding and purulent discharge图2 溃疡性结肠炎肠黏膜组织病理改变Fig.2 Pathological changes of colon tissue: H&E staining (×400)a.normal mucosal; b.mild inflammatory changes in intrinsic membrane diffuse
图 3 血清中 IL-9 表达水平Fig.3 Detection of serum IL-9 in patients with UC by ELISAA:in control group and UC patients. B:in different process of UC. C:indifferent endoscopic Mayo score. D:in different incidence type group.(*P<0.05, **P<0.01, ***P<0.001)
本文编号：2752920
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2018
【分类号】：R574.62
【图文】：
a. normal mucosal; b. hyperemia and punctate erythema; c. mucosal edema,flaky erythema; d. ulcers, blooding and purulent discharge图2 溃疡性结肠炎肠黏膜组织病理改变Fig.2 Pathological changes of colon tissue: H&E staining (×400)a.normal mucosal; b.mild inflammatory changes in intrinsic membrane diffusethe impatient chronic inflammatory cell infiltration and a crypt abscess,c.moderate inflammatory change:crypt structure change;d.severe inflammatorychanges: mucosal surface erosion, shallow ulceration and granulation tissueformation
图 1 溃疡性结肠炎内镜下 Mayo 评分Fig.1 Different performance of colonoscopya. normal mucosal; b. hyperemia and punctate erythema; c. mucosal edema,flaky erythema; d. ulcers, blooding and purulent discharge图2 溃疡性结肠炎肠黏膜组织病理改变Fig.2 Pathological changes of colon tissue: H&E staining (×400)a.normal mucosal; b.mild inflammatory changes in intrinsic membrane diffuse
图 3 血清中 IL-9 表达水平Fig.3 Detection of serum IL-9 in patients with UC by ELISAA:in control group and UC patients. B:in different process of UC. C:indifferent endoscopic Mayo score. D:in different incidence type group.(*P<0.05, **P<0.01, ***P<0.001)
【参考文献】
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1 ;Hsp90 regulates processing of NF-κB2 p100 involving protection of NF-κB-inducing kinase (NIK) from autophagy-mediated degradation[J];Cell Research;2007年06期
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