大鼠肝细胞IL-10及其受体的表达与作用
发布时间:2021-06-11 12:01
目的通过建立大鼠肝纤维化及IL-10干预模型,研究肝纤维化大鼠肝组织中IL-10的表达和变化,探讨外源性IL-10对肝细胞的作用;通过外源性IL-10对原代肝细胞进行培养干预,了解IL-10对肝细胞增殖、凋亡的影响,并分析其相关生长因子的表达变化。方法动物实验部分:建立大鼠肝纤维化和IL-10干预模型,抽提肝脏总RNA,Real-Time PCR法检测模型组与正常组IL-10表达的差异,原位Tunnel法检测IL-10对肝细胞凋亡的影响;免疫组织化学法、RT-PCR检测肝组织Bax/Bcl-2蛋白及mRNA的表达。细胞实验部分:通过胶原蛋白酶原位灌流法分离大鼠原代肝细胞, RT-PCR法检测肝内不同细胞标志基因,如:ALB、CK19、AFP、CYP8B1、CD68、CD31等基因的表达情况,并进行细胞纯度鉴定、冻存研究、复苏培养、细胞活性鉴定等;RT-PCR、Western-blot检测原代肝细胞IL-10/IL-10R1 mRNA和蛋白的表达情况;原代肝细胞分三组培养:正常组(N组,普通培养基)、对照组(C组,含胰岛素培养基)和IL-10干预组(I组,含IL-10、胰岛素的培养基),...
【文章来源】:福建医科大学福建省
【文章页数】:63 页
【学位级别】:博士
【部分图文】:
组IL-10mRNA、β-actin电泳图
组IL-10mRNA、β-actin电泳图
(0.72±0.25)%,M 组见多量阳性细胞散在(18.87±3.58)%,S 组较 N 组阳细胞数增多,两者差异显著(P<0.01);T 组阳性率(4.77±2.15)%,较 R 组(±1.85)%,阳性细胞数显著减少(19.23%,P<0.01),(图 4)。
【参考文献】:
期刊论文
[1]IGF-1对细胞凋亡的抑制调控[J]. 薛亚梅,李坤,吕杰强. 生命科学. 2007(01)
[2]原代肝细胞的分离培养及其在药物研发中的应用[J]. 罗丹,刘华钢. 广西科学. 2006(04)
[3]Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat[J]. Mei-Na Shi, Wei-Da Zheng, Li-Juan Zhang, Zhi-Xin Chen, Xiao-Zhong Wang, Department of Gastroenterology, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China. World Journal of Gastroenterology. 2005(31)
[4]Expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 in hepatic stellate cells during rat hepatic fibrosis and its intervention by IL-10[J]. Wei-Da Zheng, Li-Juan Zhang, Mei-Na Shi, Zhi-Xin Chen, Yun-Xin Chen, Yue-Hong Huang, Xiao-Zhong Wang, Department of Gastroenterology, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China. World Journal of Gastroenterology. 2005(12)
[5]慢性肝病患者血清IL-6,IL-8,IL-10含量变化[J]. 管丽华,樊必夫,高美英,李建高. 放射免疫学杂志. 2004(02)
[6]Effects of cytokines on carbon tetrachloride-induced hepatic fibrogenesis in rats[J]. Li-Juan Zhang Jie-Ping Yu Department of Gastroenterology,Renmin Hospital,Wuhan University Medical School,Wuhan 430060,Hubei Province,ChinaDan Li Yue-Hong Huang Zhi-Xin Chert Xiao-Zhong Wang Department of Gastroenterology,Union Hospital of Fujian Medical University,Fuzhou 350001,Fujian Province,China. World Journal of Gastroenterology. 2004(01)
[7]Expression of insulin-like growth factor 1 and insulin-like growth factor 1 receptor and its intervention by interleukin-10 in experimental hepatic fibrosis[J]. Xiao-Zhong Wang Zhi-Xin Chen Li-Juan Zhang Yun-Xin Chen Dan Li Feng-Lin Chen Yue-Hong Huang Department of Gastroenterology,Affiliated Union Hospital,Fujian Medical University,Fuzhou,350001,Fujian Province,China. World Journal of Gastroenterology. 2003(06)
[8]流式细胞术定量检测细胞凋亡3种方法的比较研究[J]. 郑骏年,谢叔良,陈家存,黄键. 中国免疫学杂志. 1999(10)
本文编号:3224496
【文章来源】:福建医科大学福建省
【文章页数】:63 页
【学位级别】:博士
【部分图文】:
组IL-10mRNA、β-actin电泳图
组IL-10mRNA、β-actin电泳图
(0.72±0.25)%,M 组见多量阳性细胞散在(18.87±3.58)%,S 组较 N 组阳细胞数增多,两者差异显著(P<0.01);T 组阳性率(4.77±2.15)%,较 R 组(±1.85)%,阳性细胞数显著减少(19.23%,P<0.01),(图 4)。
【参考文献】:
期刊论文
[1]IGF-1对细胞凋亡的抑制调控[J]. 薛亚梅,李坤,吕杰强. 生命科学. 2007(01)
[2]原代肝细胞的分离培养及其在药物研发中的应用[J]. 罗丹,刘华钢. 广西科学. 2006(04)
[3]Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat[J]. Mei-Na Shi, Wei-Da Zheng, Li-Juan Zhang, Zhi-Xin Chen, Xiao-Zhong Wang, Department of Gastroenterology, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China. World Journal of Gastroenterology. 2005(31)
[4]Expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 in hepatic stellate cells during rat hepatic fibrosis and its intervention by IL-10[J]. Wei-Da Zheng, Li-Juan Zhang, Mei-Na Shi, Zhi-Xin Chen, Yun-Xin Chen, Yue-Hong Huang, Xiao-Zhong Wang, Department of Gastroenterology, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China. World Journal of Gastroenterology. 2005(12)
[5]慢性肝病患者血清IL-6,IL-8,IL-10含量变化[J]. 管丽华,樊必夫,高美英,李建高. 放射免疫学杂志. 2004(02)
[6]Effects of cytokines on carbon tetrachloride-induced hepatic fibrogenesis in rats[J]. Li-Juan Zhang Jie-Ping Yu Department of Gastroenterology,Renmin Hospital,Wuhan University Medical School,Wuhan 430060,Hubei Province,ChinaDan Li Yue-Hong Huang Zhi-Xin Chert Xiao-Zhong Wang Department of Gastroenterology,Union Hospital of Fujian Medical University,Fuzhou 350001,Fujian Province,China. World Journal of Gastroenterology. 2004(01)
[7]Expression of insulin-like growth factor 1 and insulin-like growth factor 1 receptor and its intervention by interleukin-10 in experimental hepatic fibrosis[J]. Xiao-Zhong Wang Zhi-Xin Chen Li-Juan Zhang Yun-Xin Chen Dan Li Feng-Lin Chen Yue-Hong Huang Department of Gastroenterology,Affiliated Union Hospital,Fujian Medical University,Fuzhou,350001,Fujian Province,China. World Journal of Gastroenterology. 2003(06)
[8]流式细胞术定量检测细胞凋亡3种方法的比较研究[J]. 郑骏年,谢叔良,陈家存,黄键. 中国免疫学杂志. 1999(10)
本文编号:3224496
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