Kinetin Inhibits Proliferation of Hepatic Stellate Cells by

发布时间：2021-06-29 07:53

　　Liver fibrosis is an important health problem that can further progress into cirrhosis or liver cancer,and result in significant morbidity and mortality. Inhibiting proliferation and inducing apoptosis of hepatic stellate cells（HSCs） may be the key point to reverse liver fibrosis. At present,anti-fibrosis drugs are rare. Kinetin is a type of plant-derived cytokinin which has been reported to control differentiation and induce apoptosis of human cells. In this study,the HSCs were incubated with d... 

【文章来源】：Journal of Huazhong University of Science and Technology（Medical Sciences）. 2015,35(05)SCI

【文章页数】：7 页

【文章目录】：
1 MATERIALS AND METHODS
    1.1 Materials
    1.2 Culture and Passage of HSCs
    1.3 Grouping and Drug Treatment
    1.4 MTT Assay
    1.5 Counting of HSCs
    1.6 Examination of Cell Cycle by Flow Cytometry
    1.7 Detection of HSCs Apoptosis by TUNEL Method
    1.8 Detection of HSCs Apoptosis by Flow Cytometry
    1.9 Detection of Bcl-2 and Bax Expression by Immu- nohistochemistry
2 RESULTS
    2.1 Inhibitory Effects of Kinetin on Proliferation of HSCs
    2.2 Effect of Kinetin on Cell Cycle of HSCs
    2.3 Kinetin-induced Apoptosis of HSCs
    2.4 Down-regulation of Bcl-2 and Up-regulation of Bax in HSCs with Kinetin
3 DISCUSSION
Conflict of Interest Statement


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