牛磺酸对重症急性胰腺炎大鼠Kupffer细胞P38MAPK信号转导通路的影响

发布时间：2021-10-07 18:44

　　目的用牛磺酸（Taurine）对重症急性胰腺炎（severe acute pancreatitis ,SAP）大鼠进行预处理,采用联合酶原位灌注法分离枯否细胞（Kupffer cells,KCs）,检测KCs中P38-丝裂原蛋白激酶（P38 mitogen activated protein kinase, P38MAPK）的蛋白量和其活化水平以及核因子-κB（Nuclear factor-kappaB, NF-κB）的活性,探讨牛磺酸对重症急性胰腺炎大鼠KCs内P38MAPK的影响以及对分泌促炎细胞因子的作用。方法48只雄性SD大鼠采用完全随机法分为:①假手术对照组（sham operation,SO）;②重症急性胰腺炎组（SAP）;③牛磺酸预处理组（Tau）。SAP模型通过胰胆管逆行注射5%牛磺胆酸钠诱导,造模前Tau组分别于24h和12h从尾静脉内注入牛磺酸（300mmol/L,1.0ml /100g）,其它组注入等量的生理盐水。假手术或造模后分别于12h和24h处死动物,检测其血清中AST、ALT和AMS含量;然后采用联合酶原位灌注法分离KCs,采用蛋白免疫印迹法和免疫细胞化学... 

【文章来源】：重庆医科大学重庆市

【文章页数】：55 页

【学位级别】：硕士

【部分图文】：

各时间点大鼠血清中AST水平的变化

图 1-1：各时间点大鼠血清中 AST 水平的变化注： ﹡与 SO 组比较，P<0.01； ﹟与 SAP 组比较，P<0.05。Fig 1-1: The change of AST at every time in three group rats﹡P<0.01 vs SO group; ﹟P<0.05 vs SAP group.

图 1-3：各时间点大鼠血清中AMS水平的变化注： ﹡与 SO 组比较，P<0.01； ﹟与 SAP 组比较，P<0.05。Fig 1-3 :The change of AMS at every time in three group rats﹡P<0.01 vs SO group; ﹟P<0.05 vs SAP group.大鼠KCs中P38MAPK的活化情况2所示：虽然三组大鼠各时间点KCs中相对的P38MAPK的蛋白量.05），但各时间点SAP组大鼠KCs中P38MAPK明显被活化为，其明显增高，与SO组比较，差异显着(P<0.01)。用牛磺酸预处理后大下降，与SAP组相比，差异有统计学意义(P<0.05)。 2 KCs 中 P38MAPK 的蛋白量及磷酸化水平(P38MAPK/β-actin) ( )Tab2 The proteinum and phosphorylation of P38MAPK in KCs( ) SO 组 SAP 组 Tau 组12h 24h 12h 24h 12h 24h0.8399± 0.8447± 0.7539± 0.6021± 0.7506± 0.8

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