HepG2细胞中TNFα通过NF-κB信号通路下调GSTA1/GSTA4表达（英文）

发布时间：2024-06-30 04:33

　　谷胱甘肽巯基转移酶α1/α4(GSTA1/A4)是体内重要的解毒酶,可降低多种内、外源性毒性化合物的毒性.然而,胆汁淤积病人肝细胞内G STA1/A4的表达是下调的,下调机制尚不清楚.本研究通过肿瘤坏死因子α(TNFα)处理人肝癌细胞Hep G2细胞,利用实时荧光定量聚合酶链式反应(q PCR)和蛋白质印迹(Western blot)检测GSTA1/A4、核因子κB(NF-κB)和核因子E2相关因子2(Nrf2)的表达.发现TNFα在m RNA水平和蛋白质水平均抑制GSTA1/A4表达,且呈剂量与时间依赖关系.干扰NF-κB信号通路,可减弱T NFα对G STA1/A4表达的抑制作用.以上结果表明,在Hep G2细胞中,TNFα可通过激活N F-κB信号通路抑制G STA1/A4表达.

【文章页数】：9 页

【文章目录】：
1 Materials and methods
    1.1 Animal studies
    1.2 Hep G2 cells culture and treatment
    1.3 Cell viability analysis
    1.4RNA extraction and quantitative real-time polymerase chain reaction(q PCR)
    1.5Protein extraction and Western blotting analysis
    1.6 Statistical analysis
2 Results
    2.1 Reduction of Gst levels in the liver of BDL rats
    2.2 The bile acid content did not correlate with GSTA1 and GSTA4 expression in Hep G2 cells
    2.3 GSTA1 and GSTA4 expression in Hep G2cells was repressed by TNFα
    2.4 TNFαactivated NF-κB signaling and did not repress Nrf2 protein expression
    2.5 Inhibition of NF-κB activation attenuated TNFα-mediated decrease in GSTA1 and GSTA4expression
3 Discussion



本文编号：3998391