经导管射频消融去肾交感神经术对高血压犬心肌纤维化及miR-133a/CTGF的影响

发布时间：2022-02-10 07:54

　　目的：观察经导管射频消融去肾交感神经术（RDN）对高血压犬心肌纤维化程度、心肌miR-133a、结缔组织生长因子（CTGF）表达的改变,探讨RDN对心肌纤维化的影响及可能的机制。方法：1、用高脂饮食建立高血压犬模型,将28只比格犬（10-12月龄）随机分为对照组（普通饮食,n=6）和模型组（高脂饮食,n=22）。2、建模12周后将建模成功的高血压犬（n=20）随机分为手术组（n=10）及假手术组（n=10）,手术组行RDN术,假手术组仅行肾动脉造影。3、监测实验犬体重、血压,术后6月无痛处死实验犬,取心脏组织,行Masson染色观察心肌纤维化程度,计算胶原容积分数（CVF）、周围血管胶原面积比率（PVCA）, Real-time PCR检测组织miR-133a表达水平；Real-time PCR、Western-blot检测组织CTGF表达。结果：1、建模期假手术组、手术组实验犬体重、收缩压（SBP）、舒张压（DBP）、平均动脉压（MAP）均明显升高,与基线、对照组相比均有统计学差异（P<0.05）,假手术组、手术组组间对比无统计学差异（P>0.05）；2、经导管射频消融去... 

【文章来源】：中南大学湖南省211工程院校985工程院校教育部直属院校

【文章页数】：65 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
Contents
ABBREVIATION
CHAPTER 1 INTRODUCTION
CHAPTER 2 MATERIALS AND METHODS
    2.1 MATERIALS
        2.1.1 ANIMALS
        2.1.2 FEEDING
        2.1.3 IMPORTANT REAGENTS
        2.1.4 IMPORTANT EQUIPMENT AND SETTINGS
        2.1.5 MAIN REACTANTS PREPAR ATION
    2.2 METHOD OF STUDY
        2.2.1 GROUPING
        2.2.2 BLOOD PRESSURE AND BODY WEIGHT MEASUREMENT
        2.2.3 RENAL SYMPATHETIC DENERVATION INTERVENTION
        2.2.4 ECHOCARDIOGRAPHY
        2.2.5 MEASUREMENT OF HEART WEIGHT AND LVMI
        2.2.6 CARDIAC TISSUE PREPARATION
        2.2.7 MASSON STAINING OF HEART TISSUE
        2.2.8 RNA EXTRACTION
        2.2.9 DETECTION OF MIRNA WITH QPCR
        2.2.10 WESTERN BLOT
    2.3 STATISTICAL ANALYSIS
CHAPTER 3 RESULTS
    3.1 GENERAL DATA
    3.2 THE COMPARISON OF BODY WEIGHT AND BLOOD PRESSUREBETWEEN
        3.2.1 THE COMPARISON OF BODY WEIGHT BETWEEN GROUPS
        3.2.2 THE COMPARISON OF BLOOD PRESSURE BETWEEN GROUPS
    3.3 THE EFFECT OF RDN ON LVM AND LVMI
        3.3.1 THE EFFECT OF RDN ON LVM
        3.3.2 THE EFFECT OF RDN ON LVMI
    3.4 THE EFFECT OF RDN ON CARDIAC FIBROSIS
    3.5 THE EFFECT OF RDN ON MYOCARDIAL MIR-133A GENEEXPRESSION
    3.6 THE EFFECT OF RDN ON CTGF EXPRESSION
CHAPTER 4 DISCUSSION
    4.1 THE EFFECT OF RDN ON BLOOD PRESSURE
    4.2 THE EFFECT OF RDN ON CARDIAC FIBROSIS
        4.2.1 THE PATHOGENESIS OF MYOCARDIAL FIBROSIS IN HYPERTENSION
        4.2.2 RECENT ADVANCE IN THE TREATMENT OF CARDIAC REMODELING
        4.2.3 THE EFFECT OF RDN ON CARDIAC FIBROSIS
    4.3 THE POSSIBLE MECHANISM INVOLVED IN THE IMPROVEMENT OFCARDIAC FIBROSIS FOLLOWING RDN
        4.3.1 THE EXPRESSION OF MYOCARDIAL MIR-133A AND CTGF INCARDIAC FIBROSIS
        4.3.2 THE EFFECT OF RDN ON MYOCARDIAL MIR-133A-CTGF PATHWAY
    4.4 LIMITATION OF THE STUDY
CHAPTER 5 CONCLUSION
REFERENCES
REVIEW ARTICLE
    References
Acknowledgement


【参考文献】：
期刊论文
[1]自发性高血压大鼠心肌组织microRNA-133a与TGF-β1蛋白表达的改变及意义[J]. 谭文鹏,杨侃,陈晞明,陈次滨.  中国病理生理杂志. 2013(04)
[2]MicroRNA-1在心肌肥大中对L-型钙通道β2亚基的负性调控作用[J]. 吴扬,耿鹏,王玉琴,刘艳.  中国应用生理学杂志. 2012(04)



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