小分子物质和牛血清白蛋白的作用及纳米材料对其影响方面的一些研究

发布时间：2018-03-16 22:11

本文选题：血清白蛋白　切入点：纳米材料　出处：《南昌大学》2014年硕士论文　论文类型：学位论文

【摘要】：血清白蛋白(SA)是人体中一种十分重要的蛋白质,它有许多功能,包括和药物小分子的吸收结合、与食品中有害小分子的作用等。纳米材料的特有尺寸,使其相对于其它材料有很多优异的性能,并在许多领域,包括化学、医学等得到广泛的应用。而将纳米材料用作某些药物的载体是研究比较前沿和科研工作者感兴趣的课题。本论文从分子水平上研究了几种常见的小分子,包括抗癌药物白藜芦醇、烟叶中有害小分子尼古丁以及常作为补铁剂的氯化血红素与牛血清白蛋白(BSA)的作用,通过荧光光谱法等测试手段获得这几种小分子物质与BSA相作用的信息。我们还在该作用过程中引入纳米粒子,考察纳米粒子对该反应可能产生的影响,以对纳米粒子的应用作出一些有益的结论。本文的研究由三个方面组成: 1.研究了烟草中主要致烟瘾物质尼古丁与BSA二者的反应情况,并在尼古丁和BSA的反应过程中加入银纳米粒子(Ag NPs),将得到的结果与之前未加入Ag NPs时的结果进行比较,可以看到在体系中加入Ag NPs后会抑制尼古丁与BSA的结合。进而考察了Ag NPs抑制BSA与尼古丁结合的原因,运用几种光谱学方法来研究BSA与Ag NPs的结合,结果表明Ag NPs与BSA的结合会引发BSA二级结构的改变,改变了尼古丁和BSA的结合状态。 2.使用了荧光光谱、紫外吸收光谱以及微分脉冲伏安法研究补铁剂氯化血红素和BSA的作用,将得出的结果与体系中同时存在单宁酸的情况进行对比,可以看到在实验体系中加入单宁酸后,氯化血红素和BSA的结合变弱,即结合常数变小,以及结合位点数减小, BSA二级结构的改变程度也变得更弱。另一方面还研究了单宁酸对氯化血红素和BSA之间结合力的改变情况,得出单宁酸的存在并不能改变氯化血红素和BSA的结合力类型的结论。 3.首先根据文献合成聚乙二醇包裹的四氧化三铁纳米粒子(PEG-Fe3O4),并进行表征,运用荧光和紫外光谱法研究PEG-Fe3O4和抗癌药物白藜芦醇的吸附作用,结果显示纳米粒子会和白藜芦醇作用进而求得纳米粒子对白藜芦醇的载药量,随后研究了合成材料与BSA的相互作用,最后运用光谱法结合化学计量学研究了白藜芦醇与BSA的作用并考察了PEG-Fe3O4的存在对该结合作用的影响,验证了PEG-Fe3O4可以用作药物载体的可能性。
[Abstract]:Serum albumin (SA) is an important protein in the human body, it has many functions, including small molecules and drug absorption combined with food harmful to the interaction of small molecules. The unique size of nano materials, compared with the other materials have many excellent properties, and in many fields including chemistry. Medicine is widely used. And nano materials will be used as a carrier of certain drugs is the comparative study of frontiers and researchers interested in the topic. This thesis studied several common small molecules at the molecular level, including anticancer drug resveratrol, small molecule nicotine and often used as iron supplement heme chloride and bovine serum albumin harmful tobacco (BSA) role, this was the role of several small molecules and BSA phase information by means of fluorescence spectroscopy test. The process we are introducing nanoparticles The effects of nanoparticles on the reaction are investigated in order to make some useful conclusions on the application of nanoparticles. The study in this paper is composed of three aspects:
1. the study of the reaction of tobacco smoking material mainly caused by nicotine and BSA two, and joined the silver nanoparticles in the reaction process of nicotine and BSA (Ag NPs), comparing the results obtained with before joining Ag NPs results, combined with the addition of Ag NPs can be seen in the system will inhibit nicotine with BSA. In order to investigate the cause of Ag NPs inhibition of BSA in combination with nicotine, combined with BSA and Ag NPs to study the use of several spectroscopic methods, the results show that the combination of Ag NPs and BSA BSA two will lead to changes in the structure, change the binding state of nicotine and BSA.
2. using fluorescence spectroscopy, UV absorption spectroscopy and differential pulse voltammetry to study effects of iron supplement hemin and BSA, the results and the system exist at the same time compared with that of the tannic acid, tannic acid added in the experiment can be seen in the system after the heme chloride and BSA binding is weak, binding constant becomes small, and the number of binding sites decreased, change degree of BSA two level structure has become more weak. On the other hand also studied the tannic acid on the binding force between hemin and BSA changes, the presence of tannins the type of binding force does not change the conclusion of hemin and BSA.
3. according to the fe3o4nanoparticles synthesis of polyethylene glycol (PEG-Fe3O4), the package and adsorption were characterized by fluorescence and UV spectroscopy of PEG-Fe3O4 and anticancer drug resveratrol, results showed that drug loading nanoparticles and effect of resveratrol and resveratrol nanoparticles obtained, then study the interaction of synthetic materials and BSA, the last of resveratrol and BSA combined with chemometrics using spectral method and the effects of PEG-Fe3O4 on the existence with the effect, verify the possibility of PEG-Fe3O4 can be used as a drug carrier.

【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R943;O69

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