甘油二酯对2型糖尿病肾病大鼠肾脏保护机制

发布时间：2017-12-28 03:27

本文关键词：甘油二酯对2型糖尿病肾病大鼠肾脏保护机制　出处：《营养学报》2016年02期 　论文类型：期刊论文

【摘要】：目的探讨甘油二酯(DAG)对糖尿病肾病SD大鼠肾组织蛋白激酶C(PKC)表达和氧化应激的影响。方法SD雄性大鼠40只,选10只为正常对照组(I)用基础饲料喂养。另30只,采用高脂高糖饲料喂养结合链脲佐菌素(STZ)注射诱导2型糖尿病肾病模型(2型DN)。DN成模20只,均分为甘油三酯组(TAG,II)和甘油二酯两组(DAG,III)分别用TAG、DAG饲料持续喂养8w两组饲料组分(%)除TAG、DAG不同外,其余均相同,为:酪蛋白纤维素粉4,矿物质混合物3.5,维生素混合物1,淀粉61.5。实验第2、4、6、8w分别检测空腹血糖(FBG);实验结束前检测24h尿蛋白(UPRO)、血肌酐(SCR);采用苏木精-伊红(HE)染色和Massion染色观察肾脏的病理变化;采用试剂盒法检测肾皮质丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化酶(GSH)活性;免疫组化法检测肾脏中PKC的表达。结果与II组比较,III组可以显著降低模型大鼠FBG(P0.05),UPRO排泄率显著减少(P0.05),MDA含量显著下降(P0.05),肾组织病理改变减轻,并且肾组织内PKC表达下调(P0.05)。结论 DAG能改善DN大鼠肾功能,抑制氧化应激反应,下调PKC表达,延缓DN的发生发展;推测DAG保护2型糖尿病大鼠肾脏的机制与其有关。
[Abstract]:Objective to investigate the diacylglycerol (DAG) in renal tissue of diabetic nephropathy rats SD protein kinase C (PKC) expression and oxidative stress. Methods 40 male SD rats were selected and 10 were selected as the normal control group (I) to feed on the base feed. The other 30 were induced by high fat and high glucose feed combined with streptozotocin (STZ) injection to induce type 2 diabetic nephropathy model (type 2 DN). DN model 20, divided into triglyceride group (TAG, II) and two (group DAG, III diacylglycerol) respectively by TAG and DAG 8W in the two groups of continuous feeding feed feed composition (%) except TAG, DAG, the rest are the same: casein cellulose powder 4, mineral mixture 3.5 dimensional vitamin mixture 1, starch 61.5. In experiment second, fourth, sixth, 8W fasting blood glucose (FBG); before the end of the experiment, detection of 24h urinary protein (UPRO), serum creatinine (SCR); using hematoxylin eosin (HE) to observe the pathological changes of kidney and Massion staining; the renal cortex were measured by Kit Method (MDA) content, superoxide dismutase the enzyme (SOD) activity, glutathione peroxidase (GSH) activity; immunohistochemical method to detect the expression of PKC in kidney. Results compared with group II, III group could significantly reduce FBG (P0.05), UPRO excretion rate was significantly reduced (P0.05), MDA content was significantly decreased (P0.05), renal tissue pathological changes were reduced, and PKC expression in renal tissue was down regulated (P0.05). Conclusion DAG can improve renal function, inhibit oxidative stress, down regulate the expression of PKC and delay the occurrence and development of DN in DN rats. It is speculated that DAG protects the kidneys of type 2 diabetic rats.
【作者单位】：山东省农业科学院农产品研究所山东省农产品精深加工技术重点实验室;山东大学医学院公共卫生学院;
【基金】：国家自然科学基金(No.31201343) 泰山学者(No.tshw20110532)
【分类号】：R151
【正文快照】： 糖尿病肾病(DN)是一种严重的血管并发症,通常会发展成为终末期肾病。此外,DN也大大增加了心血管疾病的风险和死亡率[1]。目前,世界上DN患者90%以上的是2型DN。以往的研究证实:肾组织中氧化应激(ROS)及肌醇磷脂信号转导通路的中心环节PKC途径的激活在DN的发生发展中起重要的作

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